全谷物燕麦中的类黄酮可缓解高脂饮食诱导的小鼠高血脂症，调节胆汁酸代谢和肠道微生物群。
Flavonoids from Whole-Grain Oat Alleviated High-Fat Diet-Induced Hyperlipidemia Regulating Bile Acid Metabolism and Gut Microbiota in Mice.
机构信息
School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
出版信息
J Agric Food Chem. 2021 Jul 14;69(27):7629-7640. doi: 10.1021/acs.jafc.1c01813. Epub 2021 Jul 2.
A high-fat diet (HFD) causes hyperlipidemia, which worsens disturbances in bile acid (BA) metabolism and gut microbiota. This study aimed to investigate the regulation of flavonoids from whole-grain oat (FO) on BA metabolism and gut microbiota in HFD-induced hyperlipidemic mice. The experiment results showed that FO improved serum lipid profiles and decreased body weight and lipid deposition in HFD-fed mice. Through real-time qualitative polymerase chain reaction (RT-qPCR) and Western blot assays, by up-regulating the expression of PPARα, CPT-1, CYP7A1, FXR, TGR5, NTCP, and BSTP, and down-regulating those of SREBP-1c, FAS, and ASBT, FO suppressed lipogenesis, promoted lipolysis and BA synthesis, and efflux to faeces the FXR pathway. 16s rRNA sequencing revealed that FO significantly increased and significantly decreased , , , and . Spearman's correlation analysis showed that these bacteria were strongly correlated with hyperlipidemia-related parameters. Therefore, our results indicated that FO possessed an antihyperlipidemic effect regulating the gut-liver axis, i.e., BA metabolism and gut microbiota.
高脂肪饮食（HFD）会导致高脂血症，从而使胆汁酸（BA）代谢和肠道微生物群紊乱恶化。本研究旨在探究全燕麦麸（FO）中的类黄酮对 HFD 诱导的高脂血症小鼠 BA 代谢和肠道微生物群的调节作用。实验结果表明，FO 改善了血清脂质谱，降低了 HFD 喂养小鼠的体重和脂质沉积。通过实时定量聚合酶链反应（RT-qPCR）和 Western blot 分析，FO 通过上调 PPARα、CPT-1、CYP7A1、FXR、TGR5、NTCP 和 BSTP 的表达，下调 SREBP-1c、FAS 和 ASBT 的表达，抑制脂肪生成，促进脂肪分解和 BA 合成，并通过 FXR 途径将其排出到粪便中。16s rRNA 测序显示，FO 显著增加了 ，显著减少了 ， ， ， 。Spearman 相关性分析表明，这些细菌与与高脂血症相关的参数密切相关。因此，我们的研究结果表明，FO 具有抗高脂血症作用，可调节肠道-肝脏轴，即 BA 代谢和肠道微生物群。
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