Department of Biomedical Laboratory Science, College of Software and Digital Healthcare Convergence, Yonsei University, Wonju 26493, Korea.
Department of Food and Nutrition, Chung-Ang University, Anseong 17546, Korea.
Molecules. 2022 Jul 20;27(14):4628. doi: 10.3390/molecules27144628.
Many countries are endeavoring to strengthen the competitiveness of their biological resources by exploring and developing wild endemic plants. This study examined the effects of Nakai (FG) on the antioxidant and anti-inflammatory activity using an in vitro system. The bioactive components were also examined using chromatographic techniques. The ethanol extract of Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds. The qRT-PCR results suggested that FGE significantly downregulated the levels , , , and in LPS-stimulated RAW 264.7 cells. The FGE treatment also decreased the production of nitric oxide, TNF-α, and IL-6 significantly in a dose-dependent manner. In addition, FGE downregulated phosphorylation of MAPK and NF-κB signaling pathway-related proteins. The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities. This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
许多国家都在努力通过探索和开发野生特有植物来增强其生物资源的竞争力。本研究使用体外系统研究了那凯（FG）对抗氧化和抗炎活性的影响。还使用色谱技术检查了生物活性成分。那凯（FGE）的乙醇提取物在自由基清除和还原能力测定中表现出抗氧化活性，并且总多酚化合物含量很高。qRT-PCR 结果表明，FGE 可显著下调 LPS 刺激的 RAW 264.7 细胞中 、 、 、和 的水平。FGE 处理还可显著降低一氧化氮、TNF-α和 IL-6 的产生，呈剂量依赖性。此外，FGE 下调 MAPK 和 NF-κB 信号通路相关蛋白的磷酸化。色谱和质谱结果表明，FGE 含有生物活性类黄酮，如（+）-儿茶素、密花素、槲皮素和杨梅素，它们可能是具有抗氧化和抗炎活性的活性化合物。本研究为 FG 的抗炎活性提供了基础数据，可作为使用含生物活性类黄酮的 FGE 开发新型天然抗炎剂的良好起点。
