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虾夷扇贝无水储运过程中冷应激及干露致品质劣变调控机制研究

Study on the Regulation Mechanism of Quality Deterioration Due to Chilling Stress and Dry Exposure during Anhydrous Storage and Transportation of Yesso Scallop .

作者信息

Jiang Peihong, Chen Dongjie, Chang Xiangyang, Zhang Changfeng, Fan Xiuping, Qin Xiaoming

机构信息

College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Marine Food, Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China.

Shandong Provincial Key Laboratory of Agricultural Products Storage, Transportation and Preservation Technology, Shandong Institute of Commerce and Technology, National Engineering Research Center for Agricultural Products Logistics, Shandong Guonong Logistics Technology Co., Ltd., Jinan 250103, China.

出版信息

Foods. 2023 Jul 30;12(15):2902. doi: 10.3390/foods12152902.

DOI:10.3390/foods12152902
PMID:37569171
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10418722/
Abstract

In this paper, the quality change of Yesso scallop () in the process of anhydrous storage and transportation after cold acclimation and induced dormancy was studied, and the regulation mechanism of quality degradation during storage and transportation in the process of gradient chilling stress and drying exposure was further explored. The results show that, when transferred from hydrous to anhydrous states, the breathing pattern of the scallops changed from aerobic to anaerobic. Their gill filaments were altered and their apparent vitality constantly declined, which was reflected by the edge shrinkage of the pallium and the direct proportions of the edge reduction rate and the stimulus response period. After being in the anhydrous state for 4 d, the AEC value dropped to 67.59%. At this time, if they were placed under hydration again, the scallops resumed a good growth state. By proteomics analysis, it was revealed that cold acclimation and dry exposure mainly led to changes in biological functions and pathways, such as mitochondrial inner membrane and ATP hydrolysis activity. In addition, it can be seen from the functional annotation and enrichment analysis of the metabolite KEGG that cold acclimation promoted the purine metabolism of scallops, while dry exposure inhibited the metabolism of saturated fatty acids. In this study, the infrared sensing mode was used for the first time, too, in order to record the heart-rate changes of the scallops during circulation, which shows that non-destructive vitality monitoring of is feasible.

摘要

本文研究了冷驯化和诱导休眠后,虾夷扇贝()在无水储运过程中的品质变化,并进一步探究了梯度冷胁迫和干燥暴露过程中储运期间品质劣化的调控机制。结果表明,从有水状态转变为无水状态时,扇贝的呼吸模式从有氧变为无氧。它们的鳃丝发生改变,表观活力不断下降,这通过外套膜边缘收缩以及边缘缩减率和刺激反应期的直接比例得以体现。在无水状态下放置4 d后,AEC值降至67.59%。此时,如果再次将它们置于水化环境中,扇贝会恢复良好的生长状态。通过蛋白质组学分析发现,冷驯化和干燥暴露主要导致生物功能和途径发生变化,如线粒体内膜和ATP水解活性。此外,从代谢物KEGG的功能注释和富集分析可以看出,冷驯化促进了扇贝的嘌呤代谢,而干燥暴露抑制了饱和脂肪酸的代谢。在本研究中,还首次采用红外传感模式记录扇贝循环过程中的心率变化,这表明对虾夷扇贝进行无损活力监测是可行的。

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Fish Shellfish Immunol. 2023 Jul;138:108786. doi: 10.1016/j.fsi.2023.108786. Epub 2023 May 9.
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