Matrine Targets Intestinal to Inhibit Porcine Circovirus Type 2 Infection in Mice.

Porcine circovirus type 2 (PCV2) has caused huge economic losses to the pig industry across the world. Matrine is a natural compound that has been shown to regulate intestinal flora and has anti-PCV2 activity in mouse models. PCV2 infection can lead to changes in intestinal flora. The intestinal flora has proved to be one of the important pharmacological targets of the active components of Traditional Chinese Medicine. This study aimed to determine whether matrine exerts anti-PCV2 effects by regulating intestinal flora. In this study, fecal microbiota transplantation (FMT) was used to evaluate the effect of matrine on the intestinal flora of PCV2-infected Kunming (KM) mice. The expression of the gene in the liver and the ileum, the relative expression of mRNA, and the () gene in the ileum of mice were determined by real-time quantitative polymerase chain reaction (qPCR). ELISA was used to analyze the content of secretory immunoglobulin A (SIgA) in small intestinal fluid. was isolated and identified from the feces of KM mice in order to study its anti-PCV2 effect in vivo. The expression of the gene in the liver and the ileum and the relative expression of and mRNA in the ileum were determined by qPCR. The results showed that matrine could reduce the relative expression of mRNA by regulating intestinal flora, and that its pharmacological anti-PCV2 and effect may be related to . was successfully isolated and identified from the feces of KM mice. The in vivo experiment revealed that administration of also reduced the relative expression of mRNA, and that it had anti-PCV2 effects in PCV2-infected mice. It was found that matrine could regulate the abundance of in the gut of mice to exert an anti-PCV2 effect and inhibit PCV2-induced inflammatory response.