Department of Magnetic Materials and Devices, National Institute of Research and Development for Technical Physics, 700050 Iaşi, Romania.
Faculty of Physics, Alexandru Ioan Cuza University, 700506 Iaşi, Romania.
Int J Mol Sci. 2023 Jul 25;24(15):11916. doi: 10.3390/ijms241511916.
(1) Osteoarthritis (OA) is a progressive joint degenerative disease that currently has no cure. Limitations in the development of innovative disease modifying therapies are related to the complexity of the underlying pathogenic mechanisms. In addition, there is the unmet need for efficient drug delivery methods. Magnetic nanoparticles (MNPs) have been proposed as an efficient modality for the delivery of bioactive molecules within OA joints, limiting the side effects associated with systemic delivery. We previously demonstrated MNP's role in increasing cell proliferation and chondrogenesis. In the design of intra-articular therapies for OA, the combined NE-MNP delivery system could provide increased stability and biological effect. (2) Proprietary FeO MNPs formulated as oil-in-water (O/W) magneto nanoemulsions (MNEs) containing ascorbic acid and dexamethasone were tested for size, stability, magnetic properties, and in vitro biocompatibility with human primary adipose mesenchymal cells (ADSC), cell mobility, and chondrogenesis. In vivo biocompatibility was tested after systemic administration in mice. (3) We report high MNE colloidal stability, magnetic properties, and excellent in vitro and in vivo biocompatibility. By increasing ADSC migration potential and chondrogenesis, MNE carrying dexamethasone and ascorbic acid could reduce OA symptoms while protecting the cartilage layer.
(1) 骨关节炎（OA）是一种进行性关节退行性疾病，目前尚无治愈方法。创新疾病修饰疗法的发展受到潜在发病机制复杂性的限制。此外，还需要有效的药物输送方法。磁性纳米粒子（MNPs）已被提议作为在 OA 关节内输送生物活性分子的有效方式，限制了与全身输送相关的副作用。我们之前证明了 MNPs 在增加细胞增殖和软骨形成方面的作用。在 OA 的关节内治疗设计中，联合 NE-MNP 输送系统可以提供增加的稳定性和生物学效应。(2) 作为油包水（O/W）磁纳米乳液（MNEs）配制的专利 FeO MNPs 含有抗坏血酸和地塞米松，对其大小、稳定性、磁性能以及与人类原代脂肪间充质细胞（ADSC）的体外生物相容性、细胞迁移和软骨形成进行了测试。在小鼠中进行了系统给药后的体内生物相容性测试。(3) 我们报告了高 MNEs 胶体稳定性、磁性能和优异的体外和体内生物相容性。通过增加 ADSC 迁移潜力和软骨形成，携带地塞米松和抗坏血酸的 MNEs 可以减轻 OA 症状，同时保护软骨层。
