Chen Ge, Xu Yi, Gui Jie, Huang Yongcai, Ma Funing, Wu Wenhua, Han Te, Qiu Wenwu, Yang Liu, Song Shun
Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning 530007, China.
National Key Laboratory for Tropical Crop Breeding/Tropical Crops Genetic Resources Institute, CATAS/Germplasm Repository of Passiflora, Haikou 571101, China.
Int J Mol Sci. 2023 Jul 28;24(15):12091. doi: 10.3390/ijms241512091.
Abiotic stress is the focus of passion fruit research since it harms the industry, in which high temperature is an important influencing factor. Dof transcription factors (TFs) act as essential regulators in stress conditions. TFs can protect against abiotic stress via a variety of biological processes. There is yet to be published a systematic study of the Dof () family of passion fruit. This study discovered 13 family members by using high-quality genomes, and the members of this characterization were identified by bioinformatics. Transcriptome sequencing and qRT-PCR were used to analyze the induced expression of s under high-temperature stress during three periods, in which was significantly induced with high expression. was then chosen and converted into yeast, tobacco, and Arabidopsis, with the findings demonstrating that could significantly respond to high-temperature stress. This research lays the groundwork for a better understanding of gene regulation under high-temperature stress.
非生物胁迫是百香果研究的重点，因为它会损害该产业，其中高温是一个重要的影响因素。Dof转录因子（TFs）在胁迫条件下起关键调节作用。TFs可通过多种生物学过程抵御非生物胁迫。目前尚未有关于百香果Dof（）家族的系统研究。本研究利用高质量基因组发现了13个该家族成员，并通过生物信息学鉴定了这些成员的特征。采用转录组测序和qRT-PCR分析了三个时期高温胁迫下s的诱导表达情况，其中s被显著诱导且表达量高。然后选择s并将其转化到酵母、烟草和拟南芥中，结果表明s能显著响应高温胁迫。本研究为更好地理解高温胁迫下的基因调控奠定了基础。
