Terbojevich M, Cosani A, Palumbo M, Pregnolato F
Carbohydr Res. 1986 Jul 1;149(2):363-77. doi: 10.1016/s0008-6215(00)90058-2.
The solution properties of hyaluronic acid having various d.p. values have been examined at physiological pH and ionic strength by light-scattering techniques at various concentrations. The angular dependence of Kc/R0 was linear at low concentrations, whereas substantial curvature was present at small angles on increasing the concentration. This phenomenon was affected by the conditions of centrifugation. Moreover, the function Kc/R0 exhibited a maximum value at concentrations of polymer inversely related to the molecular weight. The maximum concentration was always higher than the critical concentration. Ageing, molecular-weight distribution, and polymer purity influenced the light-scattering response. A model is proposed in which the polysaccharide chains intertwine at relatively low concentrations of polymer and become entangled at high concentration, eventually leading to a non-homogeneous polymer network.
通过光散射技术，在生理pH值和离子强度下，对不同聚合度（d.p.）值的透明质酸在各种浓度下的溶液性质进行了研究。在低浓度时，Kc/R0的角度依赖性呈线性，而随着浓度增加，小角度处出现明显的曲率。这种现象受离心条件的影响。此外，Kc/R0函数在聚合物浓度与分子量成反比时表现出最大值。最大浓度总是高于临界浓度。老化、分子量分布和聚合物纯度会影响光散射响应。提出了一个模型，其中多糖链在聚合物浓度相对较低时相互缠绕，在高浓度时缠结，最终导致非均相聚合物网络。
