Fisheries College, Jimei University, Xiamen 361021, China.
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
Int J Mol Sci. 2023 Jul 31;24(15):12240. doi: 10.3390/ijms241512240.
The crustacean female sex hormone (CFSH) is a neurohormone peculiar to crustaceans that plays a vital role in sexual differentiation. This includes the preservation and establishment of secondary female sexual traits, as well as the inhibition of insulin-like androgenic gland factor (IAG) expression in the androgenic gland (AG). There have been no reports of CFSH receptors in crustaceans up to this point. In this study, we identified a candidate CFSH receptor from the mud crab (named -SEFIR) via protein interaction experiments and biological function experiments. Results of GST pull-down assays indicated that -SEFIR could combine with CFSH. Findings of in vitro and in vivo interference investigations exhibited that knockdown of -SEFIR could significantly induce -IAG and -STAT expression in the AG. In brief, -SEFIR is a potential CFSH receptor in , and -CFSH controls -IAG production through the CFSH-SEFIR-STAT-IAG axis.
甲壳类动物雌性激素（CFSH）是一种甲壳类动物特有的神经激素，在性别分化中起着至关重要的作用。这包括维持和建立次级雌性性特征，以及抑制在雄性腺（AG）中的胰岛素样雄激素腺因子（IAG）的表达。到目前为止，还没有关于甲壳类动物 CFSH 受体的报道。在这项研究中，我们通过蛋白质相互作用实验和生物学功能实验从泥蟹中鉴定出一个候选 CFSH 受体（命名为 -SEFIR）。GST 下拉实验结果表明，-SEFIR 可以与 CFSH 结合。体外和体内干扰研究的结果表明，-SEFIR 的敲低可以显著诱导 AG 中的 -IAG 和 -STAT 的表达。总之，-SEFIR 是 的潜在 CFSH 受体，-CFSH 通过 CFSH-SEFIR-STAT-IAG 轴控制 -IAG 的产生。
