Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao 266003, China.
Int J Mol Sci. 2023 Aug 1;24(15):12313. doi: 10.3390/ijms241512313.
is an economically important red macroalga, the cultivation of which is affected by abiotic stresses. This research intends to study the response mechanism of various components of the ubiquitin-protease pathway to abiotic stress in . The algae were treated with five common external stresses (high temperature, low temperature, O, PEG, and water shortage) to study the macroscopic and microscopic manifestations of the ubiquitin-proteasome pathway. Firstly, the changes in soluble protein and ubiquitin were detected during the five treatments, and the results showed that the content of soluble protein and ubiquitin significantly increased under most stresses. The content of the soluble protein increased the most on the second day after 20% PEG treatment, which was 1.38 times higher than that of the control group, and the content of ubiquitin increased the most 30 min after water shortage treatment, which was 3.6 times higher than that of the control group. Then, 12 key genes (, , , , , , , , , , , and ) of the ubiquitin-proteasome pathway were studied, including an estimation of the probably regulatory elements in putative promoter regions and an analysis of transcript levels. The results showed that CAAT box, LTR, GC motif, and MBS elements were present in the putative promoter regions, which might have endowed the genes with the ability to respond to stress. The transcript analysis showed that under high temperature, low temperature, PEG, O, and water shortage, all of the genes exhibited instant and significant up-regulation, and different genes had different response levels to different stresses. Many of them also showed the synergistic effect of transcript up-regulation under various stress treatments. In particular, , , , , , , and performed most significantly under the five stresses. Collectively, our exploration of the ubiquitin-proteasome pathway and the transcript levels of key genes suggest a significant role to cope with adversity, and potential candidate genes can be selected for transformation to obtain stress-resistant strains.
是一种具有重要经济价值的红色大型藻类，其栽培受非生物胁迫的影响。本研究旨在研究泛素-蛋白酶体途径的各种成分对 的非生物胁迫的响应机制。将藻类用五种常见的外部胁迫（高温、低温、O、PEG 和缺水）处理，以研究泛素-蛋白酶体途径的宏观和微观表现。首先，检测了五种处理过程中可溶性蛋白和泛素的变化，结果表明，在大多数胁迫下，可溶性蛋白和泛素的含量显著增加。在 20%PEG 处理后第二天，可溶性蛋白的含量增加最多，比对照组高 1.38 倍，在缺水处理 30 分钟后，泛素的含量增加最多，比对照组高 3.6 倍。然后，研究了泛素-蛋白酶体途径的 12 个关键基因（、、、、、、、、、、和），包括对假定启动子区域中可能的调控元件的估计和转录水平的分析。结果表明，在假定的启动子区域中存在 CAAT 盒、LTR、GC 基序和 MBS 元件，这可能使基因具有应对胁迫的能力。转录分析表明，在高温、低温、PEG、O 和缺水条件下，所有基因均表现出即刻和显著的上调，不同基因对不同胁迫的反应水平不同。许多基因在各种胁迫处理下也表现出转录上调的协同作用。特别是在五种胁迫下，、、、、、、和 表现最为显著。总的来说，我们对泛素-蛋白酶体途径和关键基因的转录水平的探索表明，该途径在应对逆境方面具有重要作用，可以选择潜在的候选基因进行转化，以获得抗胁迫菌株。
