瓜类 WUSCHEL 相关同源盒家族的全基因组鉴定和特征分析。

Genome-Wide Identification and Characterization Analysis of WUSCHEL-Related Homeobox Family in Melon ( L.).

机构信息

Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.

National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya 572000, China.

出版信息

Int J Mol Sci. 2023 Aug 1;24(15):12326. doi: 10.3390/ijms241512326.

DOI:10.3390/ijms241512326

PMID:37569702

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10419029/

Abstract

WUSCHEL-related homeobox (WOX) proteins are very important in controlling plant development and stress responses. However, the WOX family members and their role in response to abiotic stresses are largely unknown in melon ( L.). In this study, 11 WOX (CmWOX) transcript factors with conserved WUS and homeobox motif were identified and characterized, and subdivided into modern clade, ancient clade and intermediate clade based on bioinformatic and phylogenetic analysis. Evolutionary analysis revealed that the CmWOX family showed protein variations in Arabidopsis, tomato, cucumber, melon and rice. Alignment of protein sequences uncovered that all CmWOXs had the typical homeodomain, which consisted of conserved amino acids. -element analysis showed that genes may response to abiotic stress. RNA-seq and qRT-PCR results further revealed that the expression of partially genes are associated with cold and drought. and were constitutively expressed under abiotic stresses, may play a role in abiotic processes during plant development. Taken together, this study offers new perspectives on the CmWOX family's interaction and provides the framework for research on the molecular functions of genes.

摘要

WUSCHEL 相关同源盒（WOX）蛋白在植物发育和应激反应的调控中起着非常重要的作用。然而，在甜瓜（L.）中，WOX 家族成员及其在非生物胁迫响应中的作用在很大程度上是未知的。在本研究中，鉴定并表征了 11 个具有保守 WUS 和同源盒基序的 WOX（CmWOX）转录因子，并根据生物信息学和系统发育分析将其分为现代支系、古代支系和中间支系。进化分析表明，CmWOX 家族在拟南芥、番茄、黄瓜、甜瓜和水稻中表现出蛋白质变异。蛋白序列比对表明，所有 CmWOX 都具有典型的同源域，由保守氨基酸组成。- 元件分析表明，基因可能对非生物胁迫有反应。RNA-seq 和 qRT-PCR 结果进一步表明，部分基因的表达与冷胁迫和干旱胁迫有关。、在非生物胁迫下持续表达，可能在植物发育过程中的非生物过程中发挥作用。总之，本研究为 CmWOX 家族的相互作用提供了新的视角，并为研究基因的分子功能提供了框架。