LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
ALiCE-Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
Int J Mol Sci. 2023 Aug 5;24(15):12451. doi: 10.3390/ijms241512451.
Cereal-based products, which are rich in carbohydrates, are widely consumed worldwide; however, this type of food lacks other nutrients. Phenolic compounds from natural sources, such as , can be incorporated into these products to increase their nutritional and biological value. In this study, a phenolic-rich extract was obtained from leaf powder using a Soxhlet extractor. The extract obtained presented a total phenolic content of 79.0 mg of gallic acid equivalents/g and the ABTS and DPPH assays showed that the extract can act as an anti-oxidant agent, with IC values of 205.2 mg/L and 636.0 mg/L, respectively. Afterwards, fresh pasta was produced and the extract was incorporated into the pasta to improve its biological properties and extend its shelf-life. The results demonstrated that the addition of to the fresh pasta increased its anti-oxidant capacity and did not interfere with the cooking properties of the product. Moreover, the fortified pasta presented an increased shelf-life, since the extract conferred protection against microbial contamination for longer periods of time. Therefore, these findings showed that the incorporation of phenolic-rich extracts from natural sources (such as ) is a feasible sustainable biotechnological approach to produce value-added cereal-based products.
谷物类产品富含碳水化合物，在世界范围内广泛食用；然而，这类食物缺乏其他营养物质。可以将天然来源的酚类化合物（如 ）添加到这些产品中，以提高其营养价值和生物活性。在本研究中，使用索氏提取器从 叶粉中提取了一种富含酚类的提取物。所得提取物的总酚含量为 79.0 mg 没食子酸当量/g，ABTS 和 DPPH 测定表明，提取物可以作为抗氧化剂，其 IC 值分别为 205.2 mg/L 和 636.0 mg/L。之后，生产了新鲜的意大利面，并将提取物掺入到意大利面中，以提高其生物性能和延长保质期。结果表明，向新鲜意大利面中添加 可以提高其抗氧化能力，且不会影响产品的烹饪性能。此外，强化意大利面的保质期延长，因为提取物在更长的时间内提供了对微生物污染的保护。因此，这些发现表明，从天然来源（如 ）中提取富含酚类的提取物是生产附加值谷物类产品的可行可持续生物技术方法。
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