Doctoral School of Biomedical Sciences, University of Oradea, Universitatii Street, 1, 410087 Oradea, Romania.
Department of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 29 Nicolae Jiga Street, 410028 Oradea, Romania.
Molecules. 2023 Jul 28;28(15):5711. doi: 10.3390/molecules28155711.
This study aimed at the relationship between antioxidant capacity, antimicrobial activity, and in vitro evaluation of the wound healing effect of the extract obtained from Echinaceae purpureae folium (EPF). This study's objective was to assess the bioactive components (total phenol and flavonoid content) and antioxidant activity of EPF extracts using the DPPH test method. The antioxidant capacity and the quantities of the compounds with antioxidant capacity were evaluated by spectrophotometric methods. Antimicrobial activity has been investigated against various pathogenic microorganisms. The minimum inhibitory concentration was determined by the microdilution method. Additionally, our work used a scratch test to examine the in vitro wound healing effects of EPF extract on NHDF cells. Statistical analysis was used to quantify the rate of migration and proliferation of fibroblast cells within the wound. Microscope pictures of fibroblast cells exposed to various EPF extract dosages were processed to estimate the width of the wound, area of the wound, and cell density inside the wound. The study proved that there was a relationship between the antioxidant, antimicrobial, and wound healing ability of EPF extracts.
本研究旨在探讨紫松果菊叶提取物（EPF）的抗氧化能力、抗菌活性与体外伤口愈合效果之间的关系。本研究旨在采用 DPPH 试验方法评估 EPF 提取物的生物活性成分（总酚和类黄酮含量）和抗氧化活性。采用分光光度法评估抗氧化能力和具有抗氧化能力的化合物的含量。针对各种病原微生物进行了抗菌活性研究。采用微量稀释法测定最小抑菌浓度。此外，我们的工作还使用划痕试验来研究 EPF 提取物对 NHDF 细胞的体外伤口愈合作用。统计分析用于量化成纤维细胞在伤口内的迁移和增殖速度。对暴露于不同 EPF 提取物剂量的成纤维细胞的显微镜图片进行处理，以估计伤口的宽度、面积和伤口内的细胞密度。研究证明，EPF 提取物的抗氧化、抗菌和伤口愈合能力之间存在一定的关系。
