State Key Laboratory of Chinese Medicine Powder and Medicine Innovation in Hunan (Incubation), Science and Technology Innovation Center, Hunan University of Chinese Medicine, Changsha 410208, China.
Hunan Engineering Technology Research Center for Bioactive Substance Discovery of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
Molecules. 2023 Aug 4;28(15):5877. doi: 10.3390/molecules28155877.
The gas chromatography-ion mobility spectrometry (GC-IMS) method is a new technology for detecting volatile organic compounds. This study was carried out to evaluate the effects of volatile aroma compounds of Curcuma essential oils (EOs) after Co radiation by GC-IMS. Dosages of 0, 5, and 10 kGy of Co were used to analyze EOs of Curcuma after Co irradiation (named EZ-1, EZ-2, and EZ-3). The odor fingerprints of volatile organic compounds in different EOs of Curcuma samples were constructed by headspace solid-phase microextraction and GC-IMS after irradiation. The differences in odor fingerprints of EOs were compared by principal component analysis (PCA). A total of 92 compounds were detected and 65 compounds were identified, most of which were ketones, aldehydes, esters, and a small portion were furan compounds. It was found that the volatile matter content of 0 kGy and 5 kGy was closer, and the use of 10 kGy Co irradiation would have an unstable effect on the EOs. In summary, it is not advisable to use a higher dose when using Co irradiation for sterilization of Curcuma. Due to the small gradient of irradiation dose used in the experiment, the irradiation dose can be adjusted appropriately according to the required sterilization requirements during the production and storage process of Curcuma to obtain the best irradiation conditions. GC-IMS has the advantages of GC's high separation capability and IMS's fast response, high resolution, and high sensitivity, and the sample requires almost no pretreatment; it can be widely used in the analysis of traditional Chinese medicines containing volatile components. It is shown that irradiation technology has good application prospects in the sterilization of traditional Chinese medicines, but the changes in irradiation dose and chemical composition must be paid attention to.
气相色谱-离子迁移谱（GC-IMS）方法是一种检测挥发性有机化合物的新技术。本研究通过 GC-IMS 评估 Co 辐照后姜黄精油（EO）挥发性香气化合物的影响。使用 0、5 和 10 kGy 的 Co 剂量来分析 Co 辐照后的姜黄 EO（分别命名为 EZ-1、EZ-2 和 EZ-3）。通过顶空固相微萃取和 GC-IMS 对不同姜黄样品 EO 的挥发性有机化合物气味指纹进行构建，并用主成分分析（PCA）比较 EO 气味指纹的差异。共检测到 92 种化合物，鉴定出 65 种化合物，其中大部分为酮类、醛类、酯类，小部分为呋喃类化合物。结果表明，0 kGy 和 5 kGy 的挥发性物质含量更接近，而使用 10 kGy Co 辐照会对 EO 产生不稳定的影响。综上所述，在使用 Co 辐照对姜黄进行灭菌时，不建议使用更高的剂量。由于实验中使用的辐照剂量梯度较小，在姜黄的生产和储存过程中，可以根据所需的灭菌要求适当调整辐照剂量，以获得最佳的辐照条件。GC-IMS 具有 GC 高分离能力和 IMS 快速响应、高分辨率和高灵敏度的优点，且样品几乎不需要预处理；可广泛应用于含有挥发性成分的中药分析。结果表明，辐照技术在中药灭菌方面具有良好的应用前景，但必须注意辐照剂量和化学成分的变化。
