Zhou Yue-Yue, Wang You-Shao, Sun Cui-Ci, Fei Jiao
State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518121, China.
Plants (Basel). 2023 Jul 26;12(15):2772. doi: 10.3390/plants12152772.
Chitinases are believed to act as defense proteins when plants are exposed to heavy metal stress. Typical Class I chitinase genes were cloned from , , , and using the methods of reverse-transcription-polymerase chain reaction and rapid amplification of cDNA ends. All four cDNA sequences of chitinase from the mangrove plants were 1092 bp in length and consisted of an open reading frame of 831 bp, encoding 276 amino acids. However, there were differences in the sequences among the four mangrove species. Four gene proteins have a signal peptide, are located in the vacuole, and belong to the GH19 chitinase family. The sequence of chitinase was highly similar to the protein sequences of chitinases. A real-time polymerase chain reaction was used to analyze the chitinase expressions of the above four mangrove species exposed to different concentrations of heavy metal at different times. The gene expression of chitinase was higher in leaves than in other mangrove plant species. With an increase in heavy metal stress, the expression level of increased continuously. These results suggest that chitinase plays an important role in improving the heavy metal tolerance of mangrove plants.
几丁质酶被认为在植物暴露于重金属胁迫时作为防御蛋白发挥作用。采用逆转录-聚合酶链反应和cDNA末端快速扩增方法，从[具体植物名称1]、[具体植物名称2]、[具体植物名称3]和[具体植物名称4]中克隆了典型的I类几丁质酶基因。来自红树林植物的所有四个几丁质酶cDNA序列长度均为1092 bp，由一个831 bp的开放阅读框组成，编码276个氨基酸。然而，这四种红树林物种的序列存在差异。四种基因蛋白具有信号肽，位于液泡中，属于GH19几丁质酶家族。几丁质酶的序列与[具体植物名称]几丁质酶的蛋白质序列高度相似。采用实时聚合酶链反应分析上述四种红树林物种在不同时间暴露于不同浓度重金属时几丁质酶的表达情况。[具体植物名称]叶片中几丁质酶的基因表达高于其他红树林植物物种。随着重金属胁迫的增加，[具体植物名称]的表达水平持续升高。这些结果表明，几丁质酶在提高红树林植物对重金属的耐受性方面发挥着重要作用。
