Li Yuanhong, Cai Lei, Ding Ting, Tian Entang, Yan Xiaohong, Wang Xiaodong, Zhang Jiefu, Yu Kunjiang, Chen Zhuo
College of Agriculture, Guizhou University, Guiyang 550025, China.
Center for Research and Development of Fine Chemical, Guizhou University, Guiyang 550025, China.
Plants (Basel). 2023 Aug 3;12(15):2855. doi: 10.3390/plants12152855.
Rapeseed is a globally important economic crop that can be severely impacted by aphids. However, our understanding of rapeseed resistance to aphid stress is very limited. In this study, we analyzed the resistance characteristics of the low aphid-susceptible variety APL01 and the highly aphid-susceptible variety Holly in response to aphid stress. APL01 had a more significant inhibitory effect on aphid proliferation compared with Holly during the early stage of inoculation, whereas Holly showed stronger tolerance to aphid stress compared with APL01 during the later stage of inoculation. Through transcriptome, physiological, and gene expression analyses, it was revealed that chitinase activity, catalase activity, calcium signal transduction, and activation of systemic acquired resistance might be involved in aphid resistance in . The degree of inhibition of photosynthesis in plants under aphid stress directly determines the tolerance of to aphid stress. Furthermore, four promising candidate genes were screened from eight genes related to rapeseed response to biotic stress through RT-qPCR analysis of gene expression levels. These research findings represent an important step forward in understanding the resistance of rapeseed to aphid stress and provide a solid foundation for the cloning of genes responsible for this resistance.
油菜是一种全球重要的经济作物，易受到蚜虫的严重影响。然而，我们对油菜抗蚜虫胁迫的了解非常有限。在本研究中，我们分析了低感蚜品种APL01和高感蚜品种Holly对蚜虫胁迫的抗性特征。接种初期，APL01对蚜虫增殖的抑制作用比Holly更显著，而接种后期，Holly对蚜虫胁迫的耐受性比APL01更强。通过转录组、生理和基因表达分析发现，几丁质酶活性、过氧化氢酶活性、钙信号转导以及系统获得性抗性的激活可能与油菜的抗蚜性有关。蚜虫胁迫下植物光合作用的抑制程度直接决定了其对蚜虫胁迫的耐受性。此外，通过对与油菜生物胁迫响应相关的8个基因进行基因表达水平的RT-qPCR分析，筛选出了4个有前景的候选基因。这些研究结果代表了在理解油菜抗蚜虫胁迫方面向前迈出的重要一步，并为克隆负责这种抗性的基因提供了坚实的基础。
