Feng Xixian, Peng Fanjia, Yin Zujun, Wang Junjuan, Zhang Yuexin, Zhang Hong, Fan Yapeng, Xu Nan, Huang Hui, Ni Kesong, Liu Xiaoyu, Lei Yuqian, Jiang Tiantian, Wang Jing, Rui Cun, Chen Chao, Wang Shuai, Chen Xiugui, Lu Xuke, Wang Delong, Guo Lixue, Zhao Lanjie, Li Yujun, Wang Yongbo, Ye Wuwei
Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, Henan 455000, China.
Hunan Institute of Cotton Science, 3036 Shanjuan Road, Changde 415101, China.
Genomics. 2022 Jul;114(4):110398. doi: 10.1016/j.ygeno.2022.110398. Epub 2022 Jun 5.
Ca is an essential nutrient for plants and animals which plays an important role in plant signal transduction. Although the function and regulation of mechanism of Ca in alleviating various biotic and abiotic stresses in plants have been studied deeply, the molecular mechanism to adapt high Ca stress is still unclear in cotton. In this study, 103 cotton accessions were germinated under 200 mM CaCl stress, and two extremely Ca-resistant (Zhong 9807, R) and Ca-sensitive (CRI 50, S) genotypes were selected from 103 cotton accessions. The two accessions were then germinated for 5 days in 0 mM CaCl and 200 mM CaCl respectively, after which they were sampled for transcriptome sequencing. Morphological and physiological analyses suggested that PLR2 specifically expressed in R may enhance the ability of cotton to scavenge ROS by promoting the synthesis of SDG. In conclusion, this study proposed the adaptation mechanisms to response to the high Ca stress in cotton which can contribute to improve the stress resistance of cotton.
钙是动植物必需的营养元素，在植物信号转导中起重要作用。尽管钙在缓解植物各种生物和非生物胁迫中的功能及作用机制已得到深入研究，但棉花适应高钙胁迫的分子机制仍不清楚。本研究中，103份棉花种质在200 mM氯化钙胁迫下萌发，从这103份棉花种质中筛选出两个极端耐钙（中9807，R）和钙敏感（中棉所50，S）的基因型。然后将这两个种质分别在0 mM氯化钙和200 mM氯化钙中萌发5天，之后取样进行转录组测序。形态学和生理学分析表明，在R中特异性表达的PLR2可能通过促进SDG的合成来增强棉花清除活性氧的能力。总之，本研究提出了棉花响应高钙胁迫的适应机制，这有助于提高棉花的抗逆性。
