Zhai Tingting, Teng Jun, Fan Xintong, Yu Shaowei, Wang Chen, Guo Xingqi, Yang Wei, Zhang Shuxin
National Key Laboratory of Wheat Improvement, College of Life Science, Shandong Agricultural University, Tai'an 271018, China.
College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China.
Plants (Basel). 2023 Aug 3;12(15):2857. doi: 10.3390/plants12152857.
Glucosinolates and their degradation products have a wide range of actions and are important components of plant defense. (nitrile-specific protein 2) is a key regulator in the breakdown process of glucosinolates. However, the precise function of in plant disease resistance beyond its role in glucosinolate degradation is still unclear. In this study, we discovered that which was induced by DC3000, influenced genes expression and reactive oxygen burst. Additionally, omics analysis revealed that NSP2 was engaged in plant-pathogen interaction and several hormone signal transduction pathways. Furthermore, immunoprecipitation-tandem mass spectrometry analysis (IP-MS), bimolecular fluorescence complementation (BiFC), and co-immunoprecipitation demonstrated that NSP2 interacts with MPK3. Genetic analysis shows that may be a function downstream of . Upon pathogen inoculation, NSP2 protein levels increase while MPK3 protein levels decrease. Moreover, the level of phosphorylated NSP2 decreases. Taken together, this study sheds light on a new mode of synergistic action between NSP2 and MPK3 in the disease resistance process.
硫代葡萄糖苷及其降解产物具有广泛的作用，是植物防御的重要组成部分。（腈特异性蛋白2）是硫代葡萄糖苷分解过程中的关键调节因子。然而，其在植物抗病性中除了在硫代葡萄糖苷降解中的作用外的确切功能仍不清楚。在本研究中，我们发现由DC3000诱导的[具体物质未明确]影响了[相关基因未明确]基因表达和活性氧爆发。此外，组学分析表明NSP2参与植物-病原体相互作用和几种激素信号转导途径。此外，免疫沉淀-串联质谱分析（IP-MS）、双分子荧光互补（BiFC）和免疫共沉淀表明NSP2与MPK3相互作用。遗传分析表明[具体物质未明确]可能是[相关物质未明确]的下游功能。病原体接种后，NSP2蛋白水平升高而MPK3蛋白水平降低。此外，磷酸化NSP2的水平降低。综上所述，本研究揭示了NSP2和MPK3在抗病过程中协同作用的新模式。
