Iacuzzi Nicolò, Salamone Francesco, Farruggia Davide, Tortorici Noemi, Vultaggio Lorena, Tuttolomondo Teresa
Department of Agricultural, Food and Forest Sciences, Università Degli Studi di Palermo, Viale delle Scienze 13, Building 4, 90128 Palermo, Italy.
Plants (Basel). 2023 Aug 6;12(15):2877. doi: 10.3390/plants12152877.
The vegetative propagation of hops, despite being a reliable method, is not very common due to the unavailability of the plant material. In this study, the technique of in vitro propagation was applied to the Cascade variety of L. The plant material was collected from a private field in Sicily; the explants were subjected to sterilization before in vitro culture. Single-node explants were placed in in vitro culture in nine different culture media for multiplication. Thidiazuron (TDZ), Benzyladenine (BAP) and meta-Topoline (mT) were tested for multiplication phase. For the rooting phase, five types of different culture media were evaluated. Binodal cuttings coming from the previous multiplication test were placed in the culture. The rooting media differ from each other in the concentration and ratio of two auxin hormones: Indolo-3-acetic acid (IAA) and Indole-3-butyric acid (IBA). In vitro rooted plants obtained from the rooting phase were transferred to ex vitro conditions in a microbox with agri-perlite and a solution containing Murashige and Skoog (MS) basal medium at half concentration. With a culture medium containing the highest TDZ doses (H6) and combination with cytokinin (H8 and H9), the highest shoot percentage was obtained. After 3 months of in vitro culture, the highest shoot percentage was observed in the culture medium with 2 mL L of BAP. The highest rooting percentage, roots numbers and root length were found when the culture medium was supplemented with 1 mL L of IAA. The usage of agri-perlite and MS at half concentration, without PGR, allowed us to obtain a 99.1% survival rate. This micropropagation protocol is useful for obtaining virus-free plants and for the development of the brewery industry.
啤酒花的营养繁殖尽管是一种可靠的方法，但由于植物材料难以获取，并不十分常见。在本研究中，体外繁殖技术被应用于卡斯卡特品种的啤酒花。植物材料取自西西里岛的一个私人农场；外植体在进行体外培养前进行了消毒处理。单节外植体被置于九种不同的培养基中进行体外培养以增殖。噻苯隆（TDZ）、苄基腺嘌呤（BAP）和间-激动素（mT）用于增殖阶段的测试。对于生根阶段，评估了五种不同的培养基。将来自先前增殖试验的双节插条置于培养基中。生根培养基在两种生长素激素吲哚-3-乙酸（IAA）和吲哚-3-丁酸（IBA）的浓度和比例上彼此不同。从生根阶段获得的体外生根植株被转移到装有农用珍珠岩和含半浓度Murashige和Skoog（MS）基础培养基溶液的微型盒中，置于体外条件下。使用含有最高TDZ剂量（H6）并与细胞分裂素组合（H8和H9）的培养基，获得了最高的芽苗百分比。经过3个月的体外培养，在含有2 mL/L BAP的培养基中观察到最高的芽苗百分比。当培养基添加1 mL/L IAA时，发现生根百分比、根数量和根长度最高。使用农用珍珠岩和半浓度的MS，不添加植物生长调节剂，使我们获得了99.1%的成活率。这种微繁殖方案对于获得无病毒植株和啤酒酿造业的发展是有用的。
