Li Bin, Muhammad Noor, Zhang Shufeng, Lan Yunxin, Yang Yihan, Han Shoukun, Liu Mengjun, Yang Meng
College of Horticulture, Hebei Agricultural University, Baoding 071000, China.
Research Center of Chinese Jujube, Hebei Agricultural University, Baoding 071000, China.
Plants (Basel). 2023 Aug 7;12(15):2885. doi: 10.3390/plants12152885.
Jujube ( Mill.) is a commercially important tree native to China, known for its high nutritional value and widespread distribution, as well as its diverse germplasm resources. Being resilient to harsh climatic conditions, the cultivation of jujube could provide a solution to food insecurity and income for people of arid and semi-arid regions in and outside of China. The evaluation of germplasm resources and genetic diversity in jujube necessitates the use of Simple Sequence Repeat (SSR) markers. SSR markers are highly polymorphic and can be used to evaluate the genetic diversity within and between cultivars of Chinese jujube, and are important for conservation biology, breeding programs, and the discovery of important traits for Chinese jujube improvement in China and abroad. However, traditional methods of SSR development are time-consuming and inadequate to meet the growing research demands. To address this issue, we developed a novel approach called Multiple-Genome-Based SSR identification (MGB-SSR), which utilizes the genomes of three jujube cultivars to rapidly screen for polymorphic SSRs in the jujube genome. Through the screening process, we identified 12 pairs of SSR primers, which were then used to successfully classify 249 jujube genotypes. Based on the genotyping results, a digital ID card was established, enabling the complete identification of all 249 jujube plants. The MGB-SSR approach proved efficient in rapidly detecting polymorphic SSRs within the jujube genome. Notably, this study represents the first successful differentiation of jujube germplasm resources using 12 SSR markers, with 4 markers successfully identifying triploid jujube genotypes. These findings offer valuable information for the classification of Chinese jujube germplasm, thereby providing significant assistance to jujube researchers and breeders in identifying unknown jujube germplasm.
枣（Mill.）是一种原产于中国的具有重要商业价值的树种，以其高营养价值、广泛分布以及多样的种质资源而闻名。枣对恶劣气候条件具有耐受性，其种植可为中国国内外干旱和半干旱地区的人们提供粮食安全和收入解决方案。对枣种质资源和遗传多样性的评估需要使用简单序列重复（SSR）标记。SSR标记具有高度多态性，可用于评估枣品种内和品种间 的遗传多样性，对于保护生物学、育种计划以及发现国内外枣改良的重要性状具有重要意义。然而，传统的SSR开发方法耗时且不足以满足不断增长的研究需求。为了解决这个问题，我们开发了一种名为基于多基因组的SSR鉴定（MGB - SSR）的新方法，该方法利用三个枣品种的基因组快速筛选枣基因组中的多态性SSR。通过筛选过程，我们鉴定出12对SSR引物，然后用它们成功地对249个枣基因型进行了分类。基于基因分型结果，建立了数字身份证，能够完全识别所有249株枣树。MGB - SSR方法被证明在快速检测枣基因组内的多态性SSR方面是有效的。值得注意的是，本研究首次成功地利用12个SSR标记对枣种质资源进行了区分，其中4个标记成功识别了三倍体枣基因型。这些发现为枣种质分类提供了有价值的信息，从而为枣研究人员和育种者鉴定未知枣种质提供了重要帮助。
