Tao Aifen, Li Yunqing, Chen Jihan, Li Jing, Xu Jiantang, Lin Lihui, Zhang Liwu, Fang Pingping
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Fujian Key Laboratory of Crop Breeding for Design, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2024 Dec 16;13(24):3517. doi: 10.3390/plants13243517.
Roselle ( L.) simple sequence repeat (SSR) markers were developed using RNA sequencing technology, providing a foundation for genetic analysis and the identification of roselle varieties. In this study, 10 785 unigenes containing 12 994 SSR loci with an average of one SSR locus per 6.87 Kb were identified, and the occurrence frequency of the SSR loci was 11.36%. Trinucleotide repeat motifs were the most abundant, followed by dinucleotide repeats, with AAG/CTT and AT/AT being the predominant types, respectively. After screening 100 primer pairs with a polymorphic ratio of 32.0%, we obtained 32 primer pairs, resulting in clear and stable polymorphic bands. Twenty-seven primer pairs were highly or moderately polymorphic, and seven primer pairs were highly polymorphic. Genetic relationship analysis based on the selected SSR primers showed that 38 roselle accessions were classified into different clades, with those from the same regions clustered into the same subgroups. In contrast, individuals with unique morphological traits were separated. DNA fingerprints of 38 roselle varieties were constructed using five SSR primers, providing an effective method for identifying roselle varieties at a molecular level. Our data provide novel insights into the genetics of and may be used in SSR-assisted roselle breeding.
利用RNA测序技术开发了玫瑰茄（Roselle (L.)）的简单序列重复（SSR）标记，为玫瑰茄的遗传分析和品种鉴定奠定了基础。本研究共鉴定出10785个单基因，其中包含12994个SSR位点，平均每6.87 Kb有一个SSR位点，SSR位点的出现频率为11.36%。三核苷酸重复基序最为丰富，其次是二核苷酸重复，AAG/CTT和AT/AT分别是主要类型。在筛选出100对多态性比例为32.0%的引物后，获得了32对引物，产生了清晰稳定的多态性条带。27对引物具有高度或中度多态性，7对引物具有高度多态性。基于所选SSR引物的遗传关系分析表明，38份玫瑰茄种质被分为不同的分支，来自同一地区的种质聚为同一亚组。相比之下，具有独特形态特征的个体被分开。利用5对SSR引物构建了38个玫瑰茄品种的DNA指纹图谱，为在分子水平上鉴定玫瑰茄品种提供了一种有效方法。我们的数据为玫瑰茄的遗传学提供了新的见解，可用于SSR辅助的玫瑰茄育种。
