Gill Kamil, Kups Michal, Harasny Patryk, Machalowski Tomasz, Grabowska Marta, Lukaszuk Mariusz, Matuszewski Marcin, Duchnik Ewa, Fraczek Monika, Kurpisz Maciej, Piasecka Malgorzata
Department of Histology and Developmental Biology, Pomeranian Medical University, 71-210 Szczecin, Poland.
TFP Fertility Vitrolive in Szczecin, 70-483 Szczecin, Poland.
Int J Environ Res Public Health. 2021 Jun 2;18(11):5977. doi: 10.3390/ijerph18115977.
Since varicocele is so common in infertile men, this study intends to analyse the relationships between varicocele and conventional semen characteristics, sperm nuclear DNA dispersion and oxidation-reduction potential (ORP) in semen. Varicocele-positive and varicocele-negative infertile men (study groups) showed significantly lower standard sperm parameters and higher sperm DNA fragmentation (SDF) and ORP in semen than healthy volunteers and subjects with proven fertility (control groups). A lower proportion of low SDF levels (0-15% SDF) and higher incidence of high SDF levels (>30% SDF), as well as a higher prevalence of high ORP values (>1.37 mV/10 sperm/mL), were found in the study groups vs. the control groups. Moreover, infertile men had significantly lower odds ratios (ORs) for low SDF levels and significantly higher ORs for high SDF levels and high ORP. SDF and ORP were negatively correlated with sperm number, morphology, motility and vitality. Furthermore, a significant positive correlation was found between SDF and ORP. The obtained results suggest that disorders of spermatogenesis may occur in varicocele-related infertility. These abnormalities are manifested not only by reduced standard semen parameters but also by decreased sperm DNA integrity and simultaneously increased oxidative stress in semen.
由于精索静脉曲张在不育男性中非常常见，本研究旨在分析精索静脉曲张与传统精液特征、精子核DNA分散度及精液氧化还原电位（ORP）之间的关系。精索静脉曲张阳性和阴性的不育男性（研究组）与健康志愿者及已证实有生育能力的受试者（对照组）相比，精液中的标准精子参数显著更低，精子DNA碎片率（SDF）和ORP更高。与对照组相比，研究组中低SDF水平（0-15% SDF）的比例更低，高SDF水平（>30% SDF）的发生率更高，高ORP值（>1.37 mV/10精子/mL）的患病率也更高。此外，不育男性中低SDF水平的优势比（OR）显著更低，高SDF水平和高ORP的OR显著更高。SDF和ORP与精子数量、形态、活力和存活率呈负相关。此外，SDF和ORP之间存在显著正相关。所得结果表明，精索静脉曲张相关不育症可能存在精子发生障碍。这些异常不仅表现为标准精液参数降低，还表现为精子DNA完整性下降，同时精液中的氧化应激增加。
