Department of Physics and Optical Science, University of North Carolina at Charlotte, Charlotte, NC, USA.
Methods Mol Biol. 2023;2709:117-130. doi: 10.1007/978-1-0716-3417-2_7.
Cold-chain storage can be challenging and expensive for the transportation and storage of biologics, especially in low-resource settings. Nucleic acid nanoparticles (NANPs) are an example of new biological products that require refrigerated storage. Light-assisted drying (LAD) is a new processing technique to prepare biologics for anhydrous storage in a trehalose amorphous solid matrix at ambient temperatures. Small volume samples (10 μL) containing NANPs are irradiated with a 1064 nm laser to speed the evaporation of water and create an amorphous trehalose preservation matrix. In previous studies, samples were stored for 1 month at 4 °C or 20 °C without degradation. A FLIR SC655 mid-IR camera is used to record the temperature of samples during processing. The trehalose matrix was characterized using polarized light imaging to determine if crystallization occurred during processing or storage. Damage to LAD-processed NANPs was assessed after processing and storage using gel electrophoresis.
冷链储存对于生物制剂的运输和储存来说具有挑战性且成本高昂，特别是在资源匮乏的环境中。核酸纳米颗粒（NANPs）是需要冷藏储存的新型生物制品的一个例子。光辅助干燥（LAD）是一种新的加工技术，可将生物制剂制备为在海藻糖非晶固体基质中在环境温度下无水储存。含有 NANPs 的小体积样品（10 μL）用 1064nm 激光照射以加速水的蒸发并形成非晶海藻糖保存基质。在以前的研究中，样品在 4°C 或 20°C 下储存 1 个月而没有降解。FLIR SC655 中红外相机用于记录处理过程中样品的温度。使用偏光成像来表征海藻糖基质，以确定在处理或储存过程中是否发生结晶。使用凝胶电泳评估 LAD 处理后的 NANPs 处理和储存后的损伤情况。
