Department of Chemistry, Physics, Geology and the Environment, Sims Building, Winthrop University, Rock Hill, SC, USA.
Methods Mol Biol. 2023;2709:131-147. doi: 10.1007/978-1-0716-3417-2_8.
Silver and gold nanoparticle-aptamer conjugates have been extensively utilized as biosensors and microscopic vehicles that deliver a therapeutic cargo to cells. Here, we describe facile procedures to attach nucleic acid aptamers with a free thiol group to silver or gold nanoparticles. Methods to purify the nanoparticle-aptamer conjugates, verify aptamer attachment, and quantify aptamer-nanoparticle ratios are also discussed and compared. Additionally, a simple protocol that describes the aqueous synthesis of gold nanoparticles (~10 nm) is included.
银纳米粒子和金纳米粒子-适体偶联物已被广泛用作生物传感器和微载体，将治疗性货物递送到细胞。在这里，我们描述了将带有游离巯基的核酸适体连接到银或金纳米粒子上的简便方法。还讨论并比较了纯化纳米粒子-适体偶联物、验证适体连接和定量适体-纳米粒子比的方法。此外，还包括了一个简单的方案，描述了金纳米粒子（~10nm）的水相合成。
