College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, China.
State Key Laboratory of Bio-fibers and Eco-textiles, Institute of Biochemical Engineering, School of Materials Science and Engineering, Qingdao University, Qingdao, China.
Crit Rev Food Sci Nutr. 2020;60(14):2353-2368. doi: 10.1080/10408398.2019.1636763. Epub 2019 Jul 12.
Aptamers, referring to single-stranded DNA or RNA molecules can specifically recognize and bind to their targets. Based on their excellent specificity, sensitivity, high affinity, and simplicity of modification, aptamers offer great potential for pathogen detection and biomolecular screening. This article reviews aptamer screening technologies and aptamer application technologies, including gold-nanoparticle lateral flow assays, fluorescence assays, electrochemical assays, colorimetric assays, and surface-enhanced Raman assays, in the detection of foodborne pathogens. Although notable progress (more rapid, sensitive, and accurate) has been achieved in the field, challenges and drawbacks in their applications still remain to be overcome.
适体是指单链 DNA 或 RNA 分子，能够特异性地识别和结合其靶标。基于其出色的特异性、灵敏度、高亲和力和易于修饰的特点，适体在病原体检测和生物分子筛选方面具有巨大的潜力。本文综述了适体筛选技术和适体应用技术，包括金纳米粒子侧向流动分析、荧光分析、电化学分析、比色分析和表面增强拉曼分析，在食源性病原体检测中的应用。尽管在该领域已经取得了显著的进展（更快速、更灵敏、更准确），但在其应用中仍然存在挑战和缺点需要克服。
