Neuroscience Program, Department of Psychology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02453, USA.
Neuroscience Program, Department of Psychology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02453, USA.
STAR Protoc. 2023 Sep 15;4(3):102513. doi: 10.1016/j.xpro.2023.102513. Epub 2023 Aug 11.
Memory generalization is the ability to abstract knowledge from prior experiences and is critical for flexible behavior in novel situations. Here, we describe a protocol for simultaneous recording of hippocampal (area CA1)-prefrontal cortical neural ensembles in Long-Evans rats during task generalization across two distinct environments. We describe steps for building and assembling experimental apparatuses, animal preparation and surgery, and performing experiments. We then detail procedures for histology, data processing, and assessing population geometry using Uniform Manifold Approximation and Projection. For complete details on the use and execution of this protocol, please refer to Tang et al. (2023)..
记忆泛化是指从先前经验中抽象知识的能力，对于在新情境下灵活行为至关重要。在这里，我们描述了一种在长时程增强（LTP）大鼠中同时记录海马（CA1 区）-前额皮质神经集合体的方案，该方案用于在两个不同环境中进行任务泛化。我们描述了构建和组装实验设备、动物准备和手术以及进行实验的步骤。然后，我们详细介绍了使用统一流形逼近和投影进行组织学、数据处理和评估群体几何形状的过程。有关此协议的使用和执行的完整详细信息，请参阅 Tang 等人。（2023）。
