School of Public Health, Chongqing Medical University, Chongqing, 400016, China.
School of Public Health, Chongqing Medical University, Chongqing, 400016, China.
Talanta. 2024 Jan 1;266(Pt 2):125018. doi: 10.1016/j.talanta.2023.125018. Epub 2023 Aug 2.
As a reliable biomarker to evaluate the severity of sepsis, sensitive and accurate detection of procalcitonin (PCT) is essential. In this study, a dual-mode electrochemical immunosensor based on Au/ZIF-8 as substrate and Pt@CuO as signal label was constructed for the detection of PCT. By loading Au nanoparticles onto rhombic dodecahedral ZIF-8, the substrate (Au/ZIF-8) has large specific surface area and can immobilize antibody (Ab) by Au-N bonds. Meanwhile, hollow Pt@CuO nanocomposite with excellent peroxidase-like activity and electrocatalytic activity were synthesized as signal label. In the process of electrochemical testing, Pt@CuO catalyzed the reduction of hydrogen peroxide (HO) and further promotes the oxidation of hydroquinone (HQ) to achieve the synergistic amplification of electrochemical signals. The proposed immunosensor detected PCT by amperometric i-t and differential pulse voltammetry (DPV) tests with a good linear response and low limit of detection (i-t: 0.70 fg/mL and DPV: 0.40 fg/mL) in the range of 10 fg/mL∼100 ng/mL. The immunosensor exhibited excellent sensitivity and accuracy, indicating the potential application of this method for PCT detection.
作为评估脓毒症严重程度的可靠生物标志物，灵敏准确地检测降钙素原（PCT）至关重要。在这项研究中，构建了一种基于 Au/ZIF-8 作为基底和 Pt@CuO 作为信号标记物的双模式电化学免疫传感器，用于 PCT 的检测。通过将 Au 纳米粒子负载到菱面体十二面体 ZIF-8 上，基底（Au/ZIF-8）具有较大的比表面积，并可以通过 Au-N 键固定抗体（Ab）。同时，合成了具有优异过氧化物酶样活性和电催化活性的空心 Pt@CuO 纳米复合材料作为信号标记物。在电化学测试过程中，Pt@CuO 催化过氧化氢（HO）的还原，并进一步促进对苯二酚（HQ）的氧化，从而实现电化学信号的协同放大。该免疫传感器通过安培电流法（i-t）和差分脉冲伏安法（DPV）测试检测 PCT，在 10 fg/mL∼100 ng/mL 的范围内具有良好的线性响应和低检测限（i-t：0.70 fg/mL 和 DPV：0.40 fg/mL）。该免疫传感器表现出优异的灵敏度和准确性，表明该方法在 PCT 检测中的潜在应用。
