State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources College of Chemistry, Xinjiang University, Urumqi, Xinjiang 830017, China.
Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education and Xinjiang Uyghur Autonomous Region, School of Chemical Engineering and Technology, Xinjiang University, Urumqi 830017, China.
Talanta. 2024 Jun 1;273:125945. doi: 10.1016/j.talanta.2024.125945. Epub 2024 Mar 17.
Few study has been carried out on the construction of immunesensors utilized the photoelectric and catalytic signal of nanomaterial. Here, a dual-signal electrochemical immunosensor was constructed for procalcitonin (PCT) detection based on the excellent photoelectric and hydrogen evolution performance of molybdenum-doped cobalt-iron sulfur nanosheets modified by platinum nanoparticles (Pt/Mo-CoFeS). Due to the electronic structure regulation between Pt and Mo-CoFeS, Pt/Mo-CoFeS exhibits superior photoelectric and hydrogen evolution performance compared to single Mo-CoFeS, which improved the sensitivity of the electrochemical immunosensor. Furthermore, the presence of Pt improves surface area and biocompatibility, achieving more antibodies loading and signal amplification. The linear range of PCT detection are 0.002-20 ng mL and 0.002-50 ng mL, the detection limits are 0.0015 and 0.0012 ng mL. In addition, this electrochemical immunosensor was applied to the PCT analysis in human serum samples with high recoveries. F-test and t-test show that there is no significant difference in the test results between the HER and photoelectric signals, the mutual verification between above two signals can effectively improve the accuracy of detection result.
鲜有研究利用纳米材料的光电和催化信号构建免疫传感器。在这里，基于铂纳米颗粒修饰的钼掺杂钴铁硫纳米片的优异光电和析氢性能，构建了用于降钙素原（PCT）检测的双信号电化学免疫传感器。由于 Pt 和 Mo-CoFeS 之间的电子结构调节，Pt/Mo-CoFeS 表现出比单 Mo-CoFeS 更优异的光电和析氢性能，从而提高了电化学免疫传感器的灵敏度。此外，Pt 的存在提高了表面积和生物相容性，实现了更多抗体的负载和信号放大。PCT 的检测线性范围为 0.002-20ngmL 和 0.002-50ngmL，检测限分别为 0.0015 和 0.0012ngmL。此外，该电化学免疫传感器已成功应用于人血清样品中 PCT 的分析，回收率较高。F 检验和 t 检验表明，HER 和光电信号的检测结果之间没有显著差异，两种信号的相互验证可以有效提高检测结果的准确性。
