牙周炎诱导大鼠拔牙前颌骨药物相关性坏死。

Medication-related osteonecrosis of the jaw using periodontitis-induced rat before tooth extraction.

机构信息

Department of Advanced General Dentistry and Human Identification Research Institute, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.

Department of Advanced General Dentistry, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.

出版信息

BMC Oral Health. 2023 Aug 12;23(1):561. doi: 10.1186/s12903-023-03200-x.

DOI:10.1186/s12903-023-03200-x

PMID:37573298

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10422801/

Abstract

OBJECTIVE

This study aimed to investigate the occurrence of medication-related osteonecrosis of the jaw (MRONJ) after tooth extraction due to periodontitis in ovariectomized rats.

METHODS

Twenty-four osteoporosis-induced rats were administered with zoledronic acid (ZA; ZA group) or saline (CONT group). In both groups, tooth extraction was performed after inducing periodontitis, and all animals were sacrificed 8-week after tooth extraction.

RESULTS

Micro-CT of the tibia showed that the bone volume fraction, bone surface density, trabecular number, and bone mineral density were significantly higher in the ZA group than in the CONT group. Histologically, the proliferative zone on the growth plate was thicker in the ZA group than in the CONT group. Micro-CT of the extraction sites revealed that the bone volume fraction was significantly higher in the ZA group than in the CONT group. Radiologically, the ZA group showed partial healing and delayed healing. Histological analysis revealed normal bone healing status with completely healed epithelium in the extraction sites of the CONT group, whereas abnormal empty osteocytes in the necrotic bone and inflammatory infiltration were observed in the ZA group.

CONCLUSION

The incidence of MRONJ increased in the rats administered with ZA.

摘要

目的

本研究旨在探讨绝经后大鼠因牙周炎拔牙后发生药物相关性颌骨坏死（MRONJ）的情况。

方法

24 只骨质疏松症诱导大鼠给予唑来膦酸（ZA；ZA 组）或生理盐水（CONT 组）。在两组中，均在诱导牙周炎后进行拔牙，所有动物均在拔牙后 8 周处死。

结果

胫骨的 micro-CT 显示，ZA 组的骨体积分数、骨表面密度、骨小梁数量和骨矿物质密度均明显高于 CONT 组。组织学上，ZA 组的生长板增殖区比 CONT 组更厚。拔牙部位的 micro-CT 显示，ZA 组的骨体积分数明显高于 CONT 组。影像学上，ZA 组表现为部分愈合和延迟愈合。组织学分析显示，CONT 组拔牙部位的上皮完全愈合，呈现正常的骨愈合状态，而 ZA 组则观察到坏死骨中有异常的空骨细胞和炎症浸润。