Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
Poult Sci. 2023 Oct;102(10):102974. doi: 10.1016/j.psj.2023.102974. Epub 2023 Jul 27.
Pigeon paramyxovirus-1 (PPMV-1), a genetic variant of avian paramyxovirus-1 (APMV-1), has been identified in Columbiformes and is the primary cause of diseases in captive and free-ranging pigeons. However, it has also been reported that PPMV-1 can infect chickens naturally and experimentally, thus posing a potential threat to the poultry industry. This study investigated a lethal outbreak of paramyxovirus infection that occurred among 16 oriental turtle doves (Streptopelia orientalis) in a walk-in aviary at a zoo from March to April 2021. Necropsies were performed, and histopathological findings revealed mild to moderate lymphoplasmacytic infiltration in several organs, such as the pancreas, liver, kidneys, and lungs. Reverse transcription polymerase chain reaction (RT-PCR) using formalin-fixed paraffin-embedded tissue blocks, virus isolation from fresh tissue, and in situ hybridization against the fusion (F) protein confirmed the diagnosis for PPMV-1 infection. The isolated strain NTU/C239/21 was fully sequenced by next-generation sequencing, and the results of phylogenetic analyses revealed that the F protein of NTU/C239/21 shared 98.8% nucleotide sequence identity with Pigeon/Taiwan/AHRI121/2017, which was isolated from a feral pigeon in Taiwan. The present study is the first to identify PPMV-1 infection in Streptopelia orientalis and suggests that Streptopelia orientalis may also play an important role in spreading the infection, similar to pigeons in APMV-1 spreading.
鸽副黏病毒-1（PPMV-1）是禽副黏病毒-1（APMV-1）的遗传变异株，已在鸠鸽目鸟类中被鉴定出来，是笼养和自由放养鸽子疾病的主要原因。然而，也有报道称，PPMV-1 可以自然和实验感染鸡，因此对家禽业构成潜在威胁。本研究调查了 2021 年 3 月至 4 月期间，一家动物园步行式鸟舍中 16 只东方斑鸠（Streptopelia orientalis）中发生的副黏病毒感染致死性爆发。进行了剖检，组织病理学检查结果显示，胰腺、肝脏、肾脏和肺部等多个器官有轻度至中度淋巴浆细胞浸润。使用福尔马林固定石蜡包埋组织块进行逆转录聚合酶链反应（RT-PCR）、从新鲜组织中分离病毒以及针对融合（F）蛋白的原位杂交证实了 PPMV-1 感染的诊断。分离株 NTU/C239/21 通过下一代测序进行了全序列测序，系统发育分析结果表明，NTU/C239/21 的 F 蛋白与 2017 年从台湾野鸽中分离的鸽/台湾/AHRI121/2017 株的核苷酸序列同源性为 98.8%。本研究首次鉴定出东方斑鸠中的 PPMV-1 感染，并表明东方斑鸠可能与 APMV-1 传播中的鸽子一样，在传播感染方面也起着重要作用。
