Chen Guan-Shiun, Chiou Hue-Ying, Chang Yen-Chen, Liu Hao-Ping, Pan Yu-I, Chan Ming-Yun, Liu Tsung-Ching, Chia Ming-Yuan, Huang Chienjin, Chan Jacky Peng-Wen, Chang Chia-Yu
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung City 402, Taiwan.
Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung City 402, Taiwan.
Transbound Emerg Dis. 2025 Jun 25;2025:5586786. doi: 10.1155/tbed/5586786. eCollection 2025.
Bovine papillomaviruses (BPVs) are host-specific and strongly epitheliotropic infectious agents that cause benign epithelial and mucosal proliferations, with potential for malignant transformation. However, BPV1, BPV2, and BPV5 are unique in their ability to infect both epithelial and connective tissues. While BPV infections had been documented globally, there was no disease information reported from Taiwan. To investigate whether BPVs are associated with the development of cutaneous papillomas in dairy cattle in Taiwan, in the present study, eight cutaneous papilloma samples from six dairy farms were collected and analyzed by using histopathology, immunohistochemical (IHC) staining, and molecular biology methods. BPV1 and BPV2 were identified, along with a novel BPV sharing 80.9% sequence identity with BPV38. This novel BPV, classified under , was detected in both epithelial and mesenchymal cells through in situ hybridization (ISH), suggesting a broader tissue tropism than typical infections. These findings provide new insights into BPV diversity and pathogenesis.
牛乳头瘤病毒（BPVs）是宿主特异性且具有强烈亲上皮性的感染因子，可引起良性上皮和黏膜增生，并有恶变的可能。然而，BPV1、BPV2和BPV5在感染上皮组织和结缔组织的能力方面具有独特性。虽然全球都有BPV感染的记录，但台湾地区尚无相关疾病报告。为了调查BPVs是否与台湾奶牛皮肤乳头瘤的发生有关，在本研究中，从六个奶牛场收集了八个皮肤乳头瘤样本，并采用组织病理学、免疫组织化学（IHC）染色和分子生物学方法进行分析。鉴定出了BPV1和BPV2，以及一种与BPV38具有80.9%序列同一性的新型BPV。通过原位杂交（ISH）在该新型BPV在上皮细胞和间充质细胞中均被检测到，这表明其组织嗜性比典型感染更广泛。这些发现为BPV的多样性和发病机制提供了新的见解。
