Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert-Einstein-Straße 9, 07745, Jena, Germany; Institute of Physical Chemistry (IPC) and Abbe Center of Photonics (ACP), Friedrich Schiller University Jena, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Helmholtzweg 4, 07743, Jena, Germany.
Laboratorio de Bioinformática, Biología Molecular y Desarrollos Tecnológicos, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.
Talanta. 2024 Jan 1;266(Pt 2):125038. doi: 10.1016/j.talanta.2023.125038. Epub 2023 Aug 6.
Pyrazinoic acid (POA) is a metabolite of the anti-tuberculosis drug pyrazinamide (PZA), and its detection can be used to assess the resistance of Mycobacterium tuberculosis in cultures, as only sensitive strains of the bacteria can metabolize PZA into POA. Prussian blue is a well-known metal-organic framework compound widely used in various sensing platforms such as electrochemical, photochemical, and magnetic sensors. In this study, we present a novel sensing platform based on Prussian blue-modified gold nanoparticles (AuNPs) designed to enhance the affinity of POA towards the sensing surface and to capture POA molecules from aqueous solutions. This SERS-based method allows for the selective enrichment of POA, which can be detected in both pure aqueous solution and in the presence of its pro-drug PZA. The limit of detection (LOD) for POA was estimated to be 1.08 μM in pure aqueous solution and 0.18 mM in the presence of PZA. Furthermore, the precision of the SERS method was verified by the relative standard deviation (RSD) of 3.34-12.02% for three parallel samples using different matrices, i.e. aqueous solution, spiked river water and spiked simulated saliva. The recoveries of the samples ranged from 92.65 to 118.51%. These all demonstrate the potential application of the proposed detection scheme in medical research.
吡嗪酸（POA）是抗结核药物吡嗪酰胺（PZA）的代谢物，其检测可用于评估结核分枝杆菌在培养物中的耐药性，因为只有细菌的敏感株才能将 PZA 代谢为 POA。普鲁士蓝是一种众所周知的金属有机骨架化合物，广泛应用于电化学、光化学和磁传感器等各种传感平台。在本研究中，我们提出了一种基于普鲁士蓝修饰的金纳米粒子（AuNPs）的新型传感平台，旨在增强 POA 与传感表面的亲和力，并从水溶液中捕获 POA 分子。这种基于 SERS 的方法允许 POA 的选择性富集，可在纯水溶液中和其前药 PZA 的存在下检测到。在纯水溶液中，POA 的检测限（LOD）估计为 1.08 μM，在存在 PZA 的情况下为 0.18 mM。此外，通过使用不同基质的三个平行样品的相对标准偏差（RSD）为 3.34-12.02%，验证了 SERS 方法的精密度。样品的回收率范围为 92.65%至 118.51%。所有这些都表明了所提出的检测方案在医学研究中的潜在应用。
