Chávez Miriam, Escarpa Alberto
Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá, E-28802 Madrid, Spain.
Chemical Research Institute "Andrés M. Del Rio", University of Alcalá, E-28802 Madrid, Spain.
Anal Chem. 2025 Feb 11;97(5):2667-2677. doi: 10.1021/acs.analchem.4c04099. Epub 2024 Dec 24.
Here, we present three-dimensional-printed dual-channel flow-through miniaturized devices (3D) with dual electrochemical detection (ED) integrating two working electrodes each in an in-channel configuration (3D-ED). Prussian Blue (PB) shell-gold nanoparticles ((PB)AuNP) core-based electrochemistry was chosen for selective hydrogen peroxide determination. 3D-ED devices exhibited impress stability, identical intrachannel and interchannel electrochemical performances, and excellent interdevice precision with values under 9%, revealing the reliability of the design and fabrication of the devices. 3D-ED enabled long-term reliable hydrogen peroxide determination at physiological pH in Caco-2 cells under prooxidant stimulation demonstrating its outstanding electroanalytical performance. The results highlight the analytical versatility and trustworthiness of 3D-printing-based devices at miniaturized scale integrating advanced electrochemistry and its potential for real-time cell monitoring.
在此，我们展示了具有双电化学检测（ED）功能的三维打印双通道流通式微型化装置（3D），其在通道内配置中每个集成了两个工作电极（3D-ED）。基于普鲁士蓝（PB）壳-金纳米颗粒（(PB)AuNP）核的电化学方法被用于选择性测定过氧化氢。3D-ED装置表现出令人印象深刻的稳定性、通道内和通道间相同的电化学性能以及小于9%的优异装置间精密度，揭示了该装置设计和制造的可靠性。3D-ED能够在促氧化剂刺激下，在生理pH值条件下对Caco-2细胞中的过氧化氢进行长期可靠测定，证明了其出色的电分析性能。结果突出了基于3D打印的微型化装置在集成先进电化学方面的分析多功能性和可靠性及其用于实时细胞监测的潜力。
