一个β-晶状体蛋白基因HuβA3/A1（基因符号：CRYB1）定位于人类17号染色体长臂。

Localization of a beta-crystallin gene, Hu beta A3/A1 (gene symbol: CRYB1), to the long arm of human chromosome 17.

作者信息

Law M L, Cai G Y, Hartz J, Kao F T, Hogg D, Breitman M L, Tsui L C

出版信息

Cytogenet Cell Genet. 1986;42(4):202-7. doi: 10.1159/000132279.

Abstract

We have assigned a human beta-crystallin gene, Hu beta A3/A1 (gene symbol: CRYB1), to chromosome 17 using a panel of 19 human-hamster somatic cell hybrids and blot-hybridization analysis of cell hybrid DNA. Positive probe-hybridization signal was detected in a hybrid that had lost the short arm of human chromosome 17 but retained the long arm, translocated to a hamster chromosome. In addition, in situ hybridization analysis of metaphase chromosome spreads of this cell line suggested that the most probable location for CRYB1 is on the long arm of chromosome 17, in the region q21.

摘要

我们利用一组19个人-仓鼠体细胞杂种以及对细胞杂种DNA的印迹杂交分析，将一个人类β-晶体蛋白基因HuβA3/A1（基因符号：CRYB1）定位于17号染色体。在一个丢失了人类17号染色体短臂但保留了长臂并易位到一条仓鼠染色体上的杂种中，检测到了阳性探针杂交信号。此外，对该细胞系中期染色体铺展的原位杂交分析表明，CRYB1最可能的定位是在17号染色体长臂的q21区域。