人类晶状体γ-晶状体蛋白序列位于2号染色体的p12-qter区域。

Human lens gamma-crystallin sequences are located in the p12-qter region of chromosome 2.

作者信息

den Dunnen J T, Jongbloed R J, Geurts van Kessel A H, Schoenmakers J G

出版信息

Hum Genet. 1985;70(3):217-21. doi: 10.1007/BF00273445.

Abstract

The human gamma-crystallin genes constitute a multigene family whose members are only expressed in the eye lens. The chromosomal location of these sequences has been determined by screening a panel of human/rodent hybrid cell lines containing overlapping subsets of human chromosomes for the presence of human gamma-crystallin sequences. By correlating these genomic hybridization data with the chromosomal constitution of the somatic cell hybrids, all human gamma-crystallin sequences could be assigned to chromosome 2. The use of human/hamster cell hybrids derived from human Burkitt lymphoma cells carrying a reciprocal translocation between human chromosomes 2 and 8, allowed a further localization of the sequences to the region 2p12-qter.

摘要

人类γ-晶状体蛋白基因构成了一个多基因家族，其成员仅在眼晶状体中表达。通过筛选一组含有重叠人类染色体亚群的人/啮齿动物杂交细胞系，以检测人类γ-晶状体蛋白序列的存在，从而确定了这些序列的染色体定位。通过将这些基因组杂交数据与体细胞杂种的染色体组成相关联，所有人类γ-晶状体蛋白序列都可定位到2号染色体上。利用源自携带人类2号和8号染色体相互易位的人类伯基特淋巴瘤细胞的人/仓鼠细胞杂种，可将这些序列进一步定位到2p12-qter区域。