Ziaee Masumeh, Sheikhzadeh Takabi Asiyeh, Ebadollahi Asgar
Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Front Plant Sci. 2023 Jul 28;14:1187616. doi: 10.3389/fpls.2023.1187616. eCollection 2023.
Plant essential oils (EOs) can be used as a feasible tool for insect pest control. Nanoparticle formulations of plant EOs can improve the efficiency and stability of EOs, as well as insecticidal potential.
In this study, L. essential oil-loaded nanoparticles (OLNs) were prepared via an oil-in-water emulsion, followed by droplet solidiffication via ionic gelation using a cross-linker, sodium tripolyphosphate (TPP). The nanoparticles were characterized by ultraviolet and visible (UV-Vis) spectrophotometry, Fourier-transform infrared spectroscopy (FTIR), laser light scattering (LS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Moreover, the insecticidal activity of EO and OLNs was evaluated against (F.) (Coleoptera: Bostrychidae) and Jacquelin du Val. (Coleoptera: Tenebrionidae). In addition, their effectiveness was assessed on the progeny production of tested insect species.
The loading efficiency ranged from 34.33 to 84.16% when the chitosan to EO weight ratio was 1:1.25 and 1:0.5, respectively. The loading efficiency decreased with increasing EO content in the nanoparticles. The OLN particles exhibited spherical shape. The particle size in the range 120-223.6 nm and increased with the increase of EO to chitosan ratio. So that the largest mean particle size (223.6 nm) was reported in the 1:1.25 weight ratio of chitosan to the EO. The mortality percentage of and adults were 74 and 57% when exposed for 7 days to 2000 mg/kg of OLNs at the 1:1.25 weight ratio, while EO caused 62 and 44% mortality on both insect species, respectively. Therefore, OLNs can potentially improve the insecticidal activity of EO and could be applied to facilitate control of stored-product insect pests.
植物精油可作为害虫防治的一种可行工具。植物精油的纳米颗粒制剂可提高精油的效率、稳定性及杀虫潜力。
在本研究中，通过水包油乳液制备负载薰衣草精油的纳米颗粒（OLNs），随后使用交联剂三聚磷酸钠（TPP）通过离子凝胶法使液滴固化。通过紫外可见分光光度法、傅里叶变换红外光谱法（FTIR）、激光散射法（LS）、透射电子显微镜法（TEM）和扫描电子显微镜法（SEM）对纳米颗粒进行表征。此外，评估了精油和OLNs对烟草甲（鞘翅目：窃蠹科）和赤拟谷盗（鞘翅目：拟步甲科）的杀虫活性。另外，还评估了它们对受试昆虫物种后代繁殖的影响。
当壳聚糖与精油的重量比分别为1:1.25和1:0.5时，负载效率在34.33%至84.16%之间。负载效率随纳米颗粒中精油含量的增加而降低。OLN颗粒呈球形。粒径在120 - 223.6 nm范围内，并随精油与壳聚糖比例的增加而增大。因此，在壳聚糖与精油重量比为1:1.25时，报道的平均粒径最大（223.6 nm）。当以1:l.25重量比的OLNs在2000 mg/kg下暴露7天时，烟草甲和赤拟谷盗成虫的死亡率分别为74%和57%，而精油对这两种昆虫的死亡率分别为62%和44%。因此，OLNs有可能提高薰衣草精油的杀虫活性，并可用于促进对仓储害虫的防治。
