Yaowiwat Nara, Poomanee Worrapan, Leelapornpisid Pimporn, Chaiwut Phanuphong
School of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.
Green Cosmetic Technology Research Group, School of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.
ACS Omega. 2023 Jul 26;8(31):28090-28097. doi: 10.1021/acsomega.3c00602. eCollection 2023 Aug 8.
This study aimed to obtain tea flower extract (TFE) using supercritical fluid extraction, to determine the compounds present in the TFE and to establish its antioxidant activity. The fabrication of TFE nanoemulsions was also investigated using response surface methodology (RSM). UHPLC-ESI-QTOF-MS/MS and UHPLC-ESI-QqQ-MS/MS analysis showed that the TFE was composed of catechin and its derivatives, flavonols and anthocyanins, suggesting its potential as a free radical scavenger with strong reducing powers. A central composite design was applied to optimize the independent factors of the nanoemulsions. The factors had a significant ( < 0.05) effect on all response variables. The optimum level of factors for the fabrication was a surfactant-to-oil ratio of 2:1, a high hydrophilic-lipophilic balance (HLB) surfactant to low HLB surfactant ratio (HLR) of 1.6:1, and a PEG-40/PEG-60 hydrogenated castor oil ratio of 2:1. The responses obtained from the optimum levels were a 34.01 nm droplet size, a polydispersity index of 0.15, and 75.85% entrapment efficiency. In conclusion, TFE could be an antioxidant active ingredient and has been successfully loaded into nanoemulsions using RSM.
本研究旨在采用超临界流体萃取法获得茶花提取物（TFE），确定TFE中存在的化合物，并确定其抗氧化活性。还采用响应面法（RSM）研究了TFE纳米乳液的制备。超高效液相色谱-电喷雾-四极杆飞行时间串联质谱（UHPLC-ESI-QTOF-MS/MS）和超高效液相色谱-电喷雾-三重四极杆串联质谱（UHPLC-ESI-QqQ-MS/MS）分析表明，TFE由儿茶素及其衍生物、黄酮醇和花青素组成，表明其作为具有强还原能力的自由基清除剂的潜力。应用中心复合设计优化纳米乳液的独立因素。这些因素对所有响应变量均有显著影响（<0.05）。制备的最佳因素水平为表面活性剂与油的比例为2:1，高亲水亲油平衡（HLB）表面活性剂与低HLB表面活性剂的比例（HLR）为1.6:1，聚乙二醇-40/聚乙二醇-60氢化蓖麻油的比例为2:1。从最佳水平获得的响应为液滴尺寸34.01 nm、多分散指数0.15和包封率75.85%。总之，TFE可能是一种抗氧化活性成分，并且已使用RSM成功负载到纳米乳液中。
