Grzesik Michalina, Naparło Katarzyna, Bartosz Grzegorz, Sadowska-Bartosz Izabela
Department of Analytical Biochemistry, Faculty of Biology and Agriculture, University of Rzeszów, ul. Zelwerowicza 4, 35-601 Rzeszów, Poland.
Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Łódź, Pomorska 141/143, 90-236 Łódź, Poland.
Food Chem. 2018 Feb 15;241:480-492. doi: 10.1016/j.foodchem.2017.08.117. Epub 2017 Sep 1.
Antioxidant properties of five catechins and five other flavonoids were compared with several other natural and synthetic compounds and related to glutathione and ascorbate as key endogenous antioxidants in several in vitro tests and assays involving erythrocytes. Catechins showed the highest ABTS-scavenging capacity, the highest stoichiometry of Fe reduction in the FRAP assay and belonged to the most efficient compounds in protection against SIN-1 induced oxidation of dihydrorhodamine 123, AAPH-induced fluorescein bleaching and hypochlorite-induced fluorescein bleaching. Glutathione and ascorbate were less effective. (+)-catechin and (-)-epicatechin were the most effective compounds in protection against AAPH-induced erythrocyte hemolysis while (-)-epicatechin gallate, (-)-epigallocatechin gallate and (-)-epigallocatechin protected at lowest concentrations against hypochlorite-induced hemolysis. Catechins [(-)-epigallocatechin gallate and (-)-epicatechin gallate)] were most efficient in the inhibition of AAPH-induced oxidation of 2'7'-dichlorodihydroflurescein contained inside erythrocytes. Excellent antioxidant properties of catechins and other flavonoids make them ideal candidates for nanoformulations to be used in antioxidant therapy.
在涉及红细胞的多项体外试验和分析中，将5种儿茶素和5种其他类黄酮的抗氧化特性与其他几种天然和合成化合物进行了比较，并与谷胱甘肽和抗坏血酸这两种关键的内源性抗氧化剂相关联。儿茶素在ABTS清除能力方面表现最高，在FRAP分析中还原铁的化学计量比最高，并且在抵御SIN-1诱导的二氢罗丹明123氧化、AAPH诱导的荧光素漂白和次氯酸盐诱导的荧光素漂白方面属于最有效的化合物。谷胱甘肽和抗坏血酸的效果较差。（+）-儿茶素和（-）-表儿茶素在抵御AAPH诱导的红细胞溶血方面是最有效的化合物，而（-）-表儿茶素没食子酸酯、（-）-表没食子儿茶素没食子酸酯和（-）-表没食子儿茶素在最低浓度下就能保护细胞免受次氯酸盐诱导的溶血。儿茶素[（-）-表没食子儿茶素没食子酸酯和（-）-表儿茶素没食子酸酯]在抑制AAPH诱导的红细胞内2'7'-二氯二氢荧光素氧化方面最为有效。儿茶素和其他类黄酮出色的抗氧化特性使其成为用于抗氧化治疗的纳米制剂的理想候选物。
