Eid Rasha H, Aref Noha E, Ibrahim Eman S
Department of Mastitis and Neonatal Disease, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt.
Department of Microbiology and Immunology, National Research Centre, Giza, Egypt.
Vet World. 2023 May;16(5):888-894. doi: 10.14202/vetworld.2023.888-894. Epub 2023 May 7.
Bovine mastitis is a disease that affects dairy cows and impacts the global dairy industry. spp. can infect the mammary gland during lactation, intramammary treatment, or dry cow therapy. This study aimed to isolate and identify spp. in raw milk samples from cows with subclinical mastitis from dairy farms in Beheira, Giza, Alexandria, and Menoufia Governorate, Egypt. We also investigated their antibiotic sensitivity and detected the enterotoxigenic and antibiotic resistance genes.
A total of 262 milk samples (15-20 ml each) were examined microscopically, biochemically, and phenotypically. A polymerase chain reaction was used for genotypic identification and detecting antibiotic-resistance and enterotoxigenic genes. Antibiotic sensitivity was tested using the agar well diffusion test.
s was identified in 47.7% of samples. and enterotoxin genes were found in 93.64% (103/110) and 91.82% (101/110) of the samples, respectively. Tetracycline and β-lactam antibiotic-resistance genes were present in 0% (0/110) and 98.18% (108/110), respectively, of the samples. All isolates were resistant to cefepime, cefixime, and oxacillin, while they were susceptible to amoxicillin-clavulanic, chloramphenicol, ampicillin/sulbactam, and levofloxacin.
These results highlight the need to promote awareness regarding , the most common pathogen causing mastitis in Egyptian dairy cows. We also emphasized that antibiotic misuse during mastitis is a potential public health threat.
牛乳腺炎是一种影响奶牛并对全球乳制品行业产生影响的疾病。[具体菌种]在泌乳期、乳房内治疗或干奶期治疗期间可感染乳腺。本研究旨在从埃及贝赫拉、吉萨、亚历山大和米努夫省奶牛场患有亚临床乳腺炎的奶牛的生乳样本中分离和鉴定[具体菌种]。我们还研究了它们的抗生素敏感性，并检测了产肠毒素基因和抗生素抗性基因。
共检查了262份牛奶样本（每份15 - 20毫升），进行了显微镜检查、生化检查和表型检查。采用聚合酶链反应进行基因型鉴定以及检测抗生素抗性基因和产肠毒素基因。使用琼脂扩散法检测抗生素敏感性。
在47.7%的样本中鉴定出[具体菌种]。分别在93.64%（103/110）和91.82%（101/110）的样本中发现了[具体肠毒素基因1]和[具体肠毒素基因2]基因。四环素和β - 内酰胺抗生素抗性基因分别存在于0%（0/110）和98.18%（108/110）的样本中。所有分离株对头孢吡肟、头孢克肟和苯唑西林耐药，而对阿莫西林 - 克拉维酸、氯霉素、氨苄西林/舒巴坦和左氧氟沙星敏感。
这些结果凸显了提高对[具体菌种]认识的必要性，[具体菌种]是埃及奶牛乳腺炎最常见的病原体。我们还强调，乳腺炎期间抗生素的滥用是一种潜在的公共卫生威胁。
