输卵管妊娠发病机制中异位着床部位及子宫内膜组织中基因表达改变的影响：一项病例对照研究。
The Effect of Altered and Gene Expression at The Ectopic Implantation Site and Endometrial Tissues in The Tubal Pregnancy Pathogenesis: A Case-Control Study.
作者信息
Noghrehalipour Nadia, Aflatoonian Reza, Rahimipour Ali, Aghajanpour Samaneh, Najafian Aida, Chekini Zahra, Ghaffari Firouzeh, Kazerouni Faranak
机构信息
Department of Laboratory Medicine, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
出版信息
Int J Fertil Steril. 2023 Aug 7;17(4):242-247. doi: 10.22074/ijfs.2023.1972252.1390.
BACKGROUND
Ectopic pregnancy (EP) is defined as implantation and development of an embryo outside of the uterine tissue. Women undergoing assisted reproductive technologies (ART), particularly frozen embryo transfer (FET), are in high-risk populations for EP. , fibroblast growth factor-2 , and Heparin-binding epidermal growth factor genes are involved in the endometrial receptivity pathway, leading to normal eutopic implantation; Although, their relevance in the tubal pregnancy after FET is unknown. We aimed evaluation of and expression fold as endometrial receptive markers in the EP patients following the FET cycle.
MATERIALS AND METHODS
A case-control study was conducted on ten patients (five EP patients and five women in the pseudo-pregnancy group, as the control samples). Pseudo-pregnancy group was established in women who were candidates for hysterectomy for benign diseases. Fallopian tube biopsies and corresponding endometrial tissues from these patients were taken during the hysterectomy. However, the fallopian tube and endometrial tissues of EP patients were obtained during salpingectomy. The mRNA expressions of and genes in the fallopian tube and endometrial tissues were measured by real-time polymerase chain reaction (PCR) assay.
RESULTS
MUC1 mRNA expression level in the endometrium of the case group was higher than in the control group (P=0.04); however, its mRNA expression in the fallopian samples of the case group in comparison with the control group was significantly decreased (P=0.001). The mRNA expression level was not significantly different between the case and control endometrium, whereas its expression was significantly increased in the case fallopian samples compared with the control ones (P=0.001). The same pattern was observed for mRNA expression level in the fallopian samples of the case group but was significantly reduced in the endometrial samples in comparison with the control samples (P=0.03).
CONCLUSION
and gene mRNA expression changes may explain the embryo rejection from the uterus and the establishment of a receptive phenotype in fallopian cells.
背景
异位妊娠（EP）定义为胚胎在子宫组织外着床并发育。接受辅助生殖技术（ART）的女性，尤其是冷冻胚胎移植（FET）的女性，是EP的高危人群。成纤维细胞生长因子-2和肝素结合表皮生长因子基因参与子宫内膜容受性途径，导致正常的子宫内着床；然而，它们在FET后输卵管妊娠中的相关性尚不清楚。我们旨在评估FET周期后EP患者中作为子宫内膜容受性标志物的和成的表达倍数。
材料与方法
对10名患者进行了病例对照研究（5名EP患者和5名假孕组女性作为对照样本）。假孕组在因良性疾病行子宫切除术的女性中建立。这些患者的输卵管活检组织和相应的子宫内膜组织在子宫切除术中获取。然而，EP患者的输卵管和子宫内膜组织在输卵管切除术中获取。通过实时聚合酶链反应（PCR）测定法测量输卵管和子宫内膜组织中基因和成的mRNA表达。
结果
病例组子宫内膜中MUC1 mRNA表达水平高于对照组（P = 0.04）；然而，与对照组相比，其在病例组输卵管样本中的mRNA表达显著降低（P = 0.001）。病例组和对照组子宫内膜之间mRNA表达水平无显著差异，而与对照组相比，其在病例组输卵管样本中的表达显著增加（P = 0.001）。病例组输卵管样本中的mRNA表达水平也观察到相同模式，但与对照样本相比，其在子宫内膜样本中显著降低（P = 0.03）。
结论
和成基因mRNA表达变化可能解释子宫对胚胎的排斥以及输卵管细胞中容受性表型的建立。
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