Roshankhah Shiva, Barani Neda, Salahshoor Mohammad Reza, Rezakhani Leila, Khazaei Mozafar
Anatomy Department, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran. Email:
Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Int J Fertil Steril. 2023 Aug 7;17(4):287-291. doi: 10.22074/ijfs.2023.563577.1379.
Sperm chromatin abnormalities are defects in nuclear maturation and DNA integrity. These defects originated from defective spermatogenesis due to a lack of DNA repair during chromatin remodeling. Changes in semen elements can cause damage to chromatin. There is little information about the relationship between changes in trace metal elements and total antioxidant capacity (TAC) with sperm chromatin damage. The present study was conducted to determine the relationship between Selenium (Se), Iron (Fe), Zinc (Zn), Copper (Cu) and the TAC of semen with the status of human sperm chromatin.
In this experimental study, semen samples (n=30) were collected from healthy men referred to Kermanshah Motazadi Hospital and stored in liquid nitrogen; after thawing and centrifugation, sperm were separated. The atomic absorption method was used to measure the concentration of metal elements. The TAC was evaluated using the ferric-reducing antioxidant capacity of the plasma method. Furthermore, the integrity of sperm chromatin was measured using the sperm DNA fragmentation (SDF) method.
The status of sperm chromatin had a non-significant correlation with body mass index (BMI, P=0.25, r=0.21) and a non-significant negative correlation with sperm count (P=0.71, r=-0.71) and motility (P=0.75, r=0.61). In addition, there was no significant relationship between sperm chromatin and the TAC of semen (P=0.92, r=0.01). Additionally, there was no significant correlation between Se, Zn, or Cu concentration (P>0.05) and Fe concentration, which had a partially positive relationship with the chromatin state of sperm (P=0.24, r=0.20).
The trace metal elements in the seminal fluid did not play a significant role in the status of sperm chromatin.
精子染色质异常是核成熟和DNA完整性方面的缺陷。这些缺陷源于精子发生过程中的缺陷，这是由于染色质重塑过程中缺乏DNA修复所致。精液成分的变化会导致染色质受损。关于微量金属元素变化和总抗氧化能力（TAC）与精子染色质损伤之间的关系，相关信息较少。本研究旨在确定硒（Se）、铁（Fe）、锌（Zn）、铜（Cu）以及精液的TAC与人类精子染色质状态之间的关系。
在本实验研究中，从转诊至克尔曼沙赫莫塔扎迪医院的健康男性中收集精液样本（n = 30），并储存在液氮中；解冻并离心后，分离出精子。采用原子吸收法测量金属元素的浓度。使用血浆铁还原抗氧化能力法评估TAC。此外，采用精子DNA碎片化（SDF）法测量精子染色质的完整性。
精子染色质状态与体重指数（BMI，P = 0.25，r = 0.21）无显著相关性，与精子计数（P = 0.71，r = -0.71）和活力（P = 0.75，r = 0.61）呈非显著负相关。此外，精子染色质与精液的TAC之间无显著关系（P = 0.92，r = 0.01）。另外，Se、Zn或Cu浓度（P>0.05）与Fe浓度之间无显著相关性，Fe浓度与精子染色质状态呈部分正相关（P = 0.24，r = 0.20）。
精液中的微量金属元素在精子染色质状态中未发挥显著作用。
