School of Chemistry and Chemical Engineering, Yangzhou University, Jiangsu, China.
J Biomed Mater Res B Appl Biomater. 2024 Jan;112(1):e35316. doi: 10.1002/jbm.b.35316. Epub 2023 Aug 14.
As potential alternatives for calcium phosphate bone cements, magnesium phosphate bone cements (MPC) have attracted considerable attention in recent years. However, their several defects, such as rapid setting times, highly hydration temperature and alkaline pH due to the part of the unreacted phosphate, restricted their applications in human body. With aim to overcome these defects, a novel polypeptite poly(γ-glutamic acid) (γ-PGA) modified MPC were developed. Effect of γ-PGA content on the injectability, anti-washout ability, setting times, hydration temperature, mechanical compressive strength, in vitro bioactivity and degradation were investigated. Moreover, in vitro cyto-compatibility was evaluated using MC3T3-E1 cells by CCK-8 and Live/Dead staining. All these results indicated that the 10%PGA-MPC with an improved handling performances, low hydration temperature, high mechanical compressive strength, and good cyto-compatibility hold a great potential for bone repair and regeneration.
作为磷酸钙骨水泥的潜在替代品，近年来磷酸镁骨水泥（MPC）引起了广泛关注。然而，由于部分未反应的磷酸盐，它们存在凝固时间快、水化温度高、碱性 pH 值等几个缺陷，限制了其在人体中的应用。为了克服这些缺陷，开发了一种新型多肽聚（γ-谷氨酸）（γ-PGA）修饰的 MPC。研究了 γ-PGA 含量对可注射性、抗冲洗能力、凝固时间、水化温度、机械压缩强度、体外生物活性和降解的影响。此外，通过 CCK-8 和 Live/Dead 染色法，用 MC3T3-E1 细胞评估了体外细胞相容性。所有这些结果表明，具有改善的操作性能、低水化温度、高机械压缩强度和良好细胞相容性的 10%PGA-MPC 具有很大的用于骨修复和再生的潜力。
