Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Int J Biol Macromol. 2020 Oct 1;160:101-111. doi: 10.1016/j.ijbiomac.2020.05.161. Epub 2020 May 22.
Magnesium phosphate cement (MPC) can be injected to form an in situ scaffold to repair bone defects. Here we synthesized novel injectable bioactive cements (CMPCs) by incorporating different ratios of carboxymethyl chitosan (CMC, 0-10%) into MPC. The physiochemical properties, compositions, and microstructures of CMPCs were evaluated. The in vitro cellular responses of pre-osteoblast MC3T3-E1 cells to CMPCs including adhesion, proliferation, and differentiation were quantified and the underlying cellular mechanisms investigated. CMPCs had longer setting times and lower setting temperatures. CMPC injectability was enhanced by the addition of CMC. The CMPC containing 5% CMC had the highest compressive strength and washout resistance. CMPCs had a more neutral pH compared to MPC at four weeks. Furthermore, CMPC samples showed similar degradability and Mg release to MPC in Tris-HCl buffer. Osteoblasts (MC3T3-E1) showed significantly greater adherence, proliferation, and differentiation on CMPC specimens than on MPC. Finally, CMPCs effectively increased the adsorption of fibronectin and activated integrin signaling as indicated by enhanced FAK and ERK phosphorylation. Our novel CMPC composites have improved physicochemical properties and cellular responses and represent a promising material for bone regeneration.
磷酸镁水泥（MPC）可注射形成原位支架以修复骨缺损。在此，我们通过将不同比例的羧甲基壳聚糖（CMC，0-10%）掺入 MPC 中来合成新型可注射生物活性水泥（CMPC）。评估了 CMPC 的物理化学性质、组成和微观结构。定量研究了前成骨细胞 MC3T3-E1 细胞对 CMPC 的体外细胞反应，包括粘附、增殖和分化，并研究了其潜在的细胞机制。CMPC 的凝固时间更长，凝固温度更低。通过添加 CMC 可提高 CMPC 的可注射性。含 5%CMC 的 CMPC 具有最高的抗压强度和耐洗脱性。与 MPC 相比，CMPC 在四周时具有更中性的 pH 值。此外，在 Tris-HCl 缓冲液中，CMPC 样品的降解性和 Mg 释放与 MPC 相似。成骨细胞（MC3T3-E1）在 CMPC 标本上的粘附、增殖和分化明显优于 MPC。最后，CMPC 有效增加了纤维连接蛋白的吸附，并通过增强 FAK 和 ERK 磷酸化激活整合素信号。我们的新型 CMPC 复合材料具有改善的物理化学性质和细胞反应，是一种有前途的骨再生材料。
