State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.
Department of Clinical Laboratory, Air Force Medical Center, Beijing, PR China.
J Med Microbiol. 2023 Aug;72(8). doi: 10.1099/jmm.0.001741.
The human oocyte microenvironment is follicular fluid, which is important for follicle growth, ovulation and maturation of the oocyte. The micro-organisms present in follicular fluid could be a predictor of fertilization outcomes. Women with follicular fluid colonized with micro-organisms can be asymptomatic, but the presence of some genera in the follicular fluid correlates with fertilization. To confirm the existence of micro-organisms in follicular fluid, and to profile the micro-organisms present in follicular fluid sampled from women undergoing fertilization with different outcomes. Women undergoing fertilization (=163) were divided into different subgroups according to their fertilization outcomes. Their follicular fluid samples were collected, and among them, 157 samples were analysed by 16S rDNA sequencing, and 19 samples were analysed using culturomics. The culturomics results suggested that the 19 follicular fluid samples were not sterile. The isolation rates for , and were >50 % in the 19 samples. Linear discriminant analysis effect size analysis showed differential bacteria abundance according to the pregnancy rate, the rate of normal fertilization, the rate of high-quality embryos and the rate of available oocytes. The sequencing results showed that micro-organisms could be detected in all 157 samples. , , , and were detected in all of the samples, but with a wide range of relative abundance. , , and constituted a notable fraction of the microbiota. Follicular fluid is not sterile. Micro-organisms in follicular fluid could be a predictor of fertilization outcomes.
人类卵母细胞的微环境是卵泡液，它对卵泡生长、排卵和卵母细胞成熟至关重要。卵泡液中存在的微生物可能是受精结局的预测因素。卵泡液中存在微生物的女性可能无症状，但某些属的存在与受精有关。为了确认卵泡液中存在微生物，并分析不同受精结局的女性卵泡液中存在的微生物特征。根据受精结局将 163 名接受受精的女性分为不同亚组。采集她们的卵泡液样本，其中 157 个样本进行 16S rDNA 测序分析，19 个样本进行培养组学分析。培养组学结果表明，19 个卵泡液样本并非无菌。在这 19 个样本中， 、 和 的分离率>50%。线性判别分析效应量分析显示，根据妊娠率、正常受精率、优质胚胎率和可利用卵母细胞率，细菌丰度存在差异。测序结果表明，所有 157 个样本中均可检测到微生物。所有样本均检测到 、 、 、 ，但相对丰度范围较宽。 、 、 和 构成了微生物群的显著部分。卵泡液不是无菌的。卵泡液中的微生物可能是受精结局的预测因素。
