Center for Tissue Engineering and Stem Cell Research, Guizhou Medical University, Guiyang, Guizhou, China.
NHC Key Laboratory of Carcinogenesis, Cancer Research Institute and School of Basic Medicine, Central South University, Changsha, Hunan, China.
Methods Mol Biol. 2023;2712:19-27. doi: 10.1007/978-1-0716-3433-2_3.
Ferroptosis is characterized by the accumulation of lipid peroxidation driven by iron. As a regulated cell death, ferroptosis plays a critical role in various diseases and exhibits great therapeutic potentials. However, the mechanisms underlying ferroptosis, including its occurrence, execution, and regulation, remain poorly understood, which is necessary for developing effective therapeutic strategies. In this chapter, we summarize chromatin immunoprecipitation (ChIP) assay for the research of proteins-chromatin interactions. Moreover, Chromatin Isolation by RNA Purification (ChIRP) trial is introduced to investigate the interactions between lncRNA and chromatin. The application of ChIP and ChIRP is expected to explore the transcription and epigenetic regulation of ferroptosis deeply for therapeutic benefits.
铁死亡的特征是铁驱动的脂质过氧化积累。作为一种受调控的细胞死亡方式，铁死亡在各种疾病中发挥着关键作用，并具有巨大的治疗潜力。然而，铁死亡的机制，包括其发生、执行和调控，仍知之甚少，这对于开发有效的治疗策略是必要的。在这一章中，我们总结了染色质免疫沉淀（ChIP）实验用于研究蛋白质与染色质的相互作用。此外，还介绍了 RNA 纯化染色质免疫沉淀（ChIRP）实验，以研究 lncRNA 与染色质之间的相互作用。ChIP 和 ChIRP 的应用有望深入研究铁死亡的转录和表观遗传调控，以带来治疗益处。
