Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Hunan Key Laboratory of Viral Hepatitis, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Methods Mol Biol. 2023;2712:211-222. doi: 10.1007/978-1-0716-3433-2_19.
Ferroptosis is a type of regulated cell death driven by oxidative damage, characterized by iron overload and lipid peroxidation, and regulated by a network of distinct molecules and organelles. Impaired ferroptotic response is implicated in multiple physiological and pathological processes, including tumorigenesis, neurodegeneration, and ischemia-reperfusion damage. Classical techniques of immunohistochemistry (IHC) and immunofluorescence (IF) can be employed to exhibit antigen expression and location in tissues observed with microscopy, making them powerful tools in studying the ferroptosis process. In this chapter, we introduce commonly used protocols and summarize typical markers used in IHC and IF to monitor ferroptosis.
铁死亡是一种由氧化损伤驱动的受调控的细胞死亡方式，其特征是铁过载和脂质过氧化，并且受一系列不同的分子和细胞器的调控。铁死亡反应受损与多种生理和病理过程有关，包括肿瘤发生、神经退行性变和缺血再灌注损伤。免疫组织化学（IHC）和免疫荧光（IF）等经典技术可用于通过显微镜观察展示组织中抗原的表达和位置，因此它们是研究铁死亡过程的有力工具。在本章中，我们介绍了常用的方案，并总结了 IHC 和 IF 中用于监测铁死亡的典型标志物。
