Department of Biochemistry and Molecular Biology, Capital Medical University, Beijing, China.
Department of Neurology, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Methods Mol Biol. 2023;2712:233-251. doi: 10.1007/978-1-0716-3433-2_21.
Ferroptosis is a regulated form of non-apoptotic cell death driven by iron-dependent lipid peroxidation. In the past decade, ferroptosis has been reported to be involved in the pathological role in the central nervous system degenerative diseases (e.g., Alzheimer's disease, Huntington's disease, and Parkinson's disease), stroke, traumatic brain injury, and brain tumor. However, how to reliably detect and classify ferroptosis from other cell death in pathological conditions remains a great challenge, especially in primary brain cells and brain tissues. Here, we summarize the methods and protocols (such as real-time PCR, western blotting, immunofluorescence staining, lipid peroxidation assay kits and probe, immunofluorescence staining, GPX activity and glutathione depletion assay kits, iron detection, and TEM) used in the present study to detect and classify ferroptosis in the brain.
铁死亡是一种由铁依赖性脂质过氧化驱动的调节性非细胞凋亡形式的细胞死亡。在过去的十年中，铁死亡被报道参与了中枢神经系统退行性疾病（如阿尔茨海默病、亨廷顿病和帕金森病）、中风、创伤性脑损伤和脑肿瘤的病理作用。然而，如何在病理条件下可靠地检测和分类铁死亡与其他细胞死亡仍然是一个巨大的挑战，特别是在原代脑细胞和脑组织中。在这里，我们总结了目前用于检测和分类大脑中铁死亡的方法和方案（如实时 PCR、western blot、免疫荧光染色、脂质过氧化检测试剂盒和探针、免疫荧光染色、GPX 活性和谷胱甘肽耗竭检测试剂盒、铁检测和 TEM）。
