激活 NLRP1 炎性小体的方法。
Methods to Activate the NLRP1 Inflammasome.
机构信息
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
出版信息
Methods Mol Biol. 2023;2696:211-222. doi: 10.1007/978-1-0716-3350-2_14.
In addition to being the first NLR protein proposed to form inflammasome, NLRP1s have attracted much attention in their activation mechanism by post-translational auto-proteolysis to generate C-terminal CARD containing fragment to form inflammasome. Among NLRP1, mouse NLRP1B but not human NLRP1 is well studied for its activation by lethal toxin. As dissecting the cellular components involved in NLRP1-associated diseases is highly dependent on NLRP1 inflammasome activation, experiments that can lead to NLRP1 activation is of pivotal importance to elucidate the biological role and the activation mechanism of NLRP1 especially in human. In this chapter we describe methods commonly used for mouse NLRP1B inflammasome activation as well as activation of human NLRP1 inflammasome visualized by ASC speck formation in our laboratory.
除了作为第一个被提议形成炎性小体的 NLR 蛋白，NLRP1s 在其通过翻译后自动切割产生含有 C 端 CARD 的片段以形成炎性小体的激活机制方面引起了广泛关注。在 NLRP1 中，由于其被致死毒素激活的机制已被很好地研究，因此鼠 NLRP1B 而非人 NLRP1 受到了更多关注。由于解析与 NLRP1 相关疾病相关的细胞成分高度依赖于 NLRP1 炎性小体的激活，因此能够导致 NLRP1 激活的实验对于阐明 NLRP1 的生物学作用及其激活机制至关重要，尤其是在人类中。在本章中，我们描述了在我们实验室中常用的用于激活鼠 NLRP1B 炎性小体以及通过 ASC 斑点形成可视化激活人 NLRP1 炎性小体的方法。
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