Institute of Virology, Wenzhou University, Wenzhou, 325035, China.
The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China.
Braz J Microbiol. 2023 Sep;54(3):2437-2443. doi: 10.1007/s42770-023-01092-1. Epub 2023 Aug 14.
Sus scrofa papillomatosis (SsP) is a tumour caused by Sus scrofa papillomaviruses (SsPVs). To investigate the presence of SsPVs in China, 354 domestic pig skin samples collected from Guangxi Province were examined for SsPV DNA by PCR. Three SsPV1s (GX12, GX14, and GX18) were identified with a prevalence of 0.847% (3/354). Sequence analysis showed that L1 of SsPV1/GX12 and SsPV1/GX14 had 99.7% and 99.6% nucleotide identify with the reference SsPV1a, respectively. Phylogenetic and evolutionary analyses showed that SsPV1/GX12 and SsPV1/14 clustered into SsPV1a and that SsPV1/GX18 clustered into SsPV1b. Compared with other SsPV L1 and L2 proteins, we found that the SsPV1/GX18 and SsPV1b strains shared the same unique substitutions, and SsPV1/GX12, SsPV1/GX14, and SsPV1a shared almost identical amino acid sequences. This study reports the first detection of SsPV DNA in China based on whole genome information and provides a scientific basis for the development of SsPV pathogenic biology, epidemiology, and prevention, as well as control technology research.
猪乳头状瘤病（SsP）是由猪乳头状瘤病毒（SsPVs）引起的肿瘤。为了调查中国是否存在 SsPVs，本研究采用 PCR 方法对从广西采集的 354 份家猪皮肤样本进行了 SsPV DNA 检测。鉴定出 3 株 SsPV1（GX12、GX14 和 GX18），阳性率为 0.847%（3/354）。序列分析表明，SsPV1/GX12 和 SsPV1/GX14 的 L1 核苷酸与参考 SsPV1a 的同源性分别为 99.7%和 99.6%。系统进化和遗传进化分析表明，SsPV1/GX12 和 SsPV1/GX14 聚类于 SsPV1a 分支，SsPV1/GX18 聚类于 SsPV1b 分支。与其他 SsPV L1 和 L2 蛋白相比，我们发现 SsPV1/GX18 和 SsPV1b 株具有相同的独特取代，而 SsPV1/GX12、SsPV1/GX14 和 SsPV1a 株具有几乎相同的氨基酸序列。本研究基于全基因组信息首次报道了中国 SsPV DNA 的检测，为 SsPV 致病生物学、流行病学和预防以及控制技术研究提供了科学依据。
