Gheit Tarik, Dutta Sankhadeep, Oliver Javier, Robitaille Alexis, Hampras Shalaka, Combes Jean-Damien, McKay-Chopin Sandrine, Le Calvez-Kelm Florence, Fenske Neil, Cherpelis Basil, Giuliano Anna R, Franceschi Silvia, McKay James, Rollison Dana E, Tommasino Massimo
International Agency for Research on Cancer, World Health Organization, Lyon 69372, France.
Department of Cancer Epidemiology, Moffitt Cancer Center, Tampa, FL, USA.
Virology. 2017 Jun;506:45-54. doi: 10.1016/j.virol.2017.03.007. Epub 2017 Mar 22.
The small double-stranded DNA polyomaviruses (PyVs) form a family of 73 species, whose natural hosts are primarily mammals and birds. So far, 13 PyVs have been isolated in humans, and some of them have clearly been associated with several diseases, including cancer. In this study, we describe the isolation of a novel PyV in human skin using a sensitive degenerate PCR protocol combined with next-generation sequencing. The new virus, named Lyon IARC PyV (LIPyV), has a circular genome of 5269 nucleotides. Phylogenetic analyses showed that LIPyV is related to the raccoon PyV identified in neuroglial tumours in free-ranging raccoons. Analysis of human specimens from cancer-free individuals showed that 9 skin swabs (9/445; 2.0%), 3 oral gargles (3/140; 2.1%), and one eyebrow hair sample (1/439; 0.2%) tested positive for LIPyV. Future biological and epidemiological studies are needed to confirm the human tropism and provide insights into its biological properties.
小型双链DNA多瘤病毒（PyVs）构成了一个包含73个物种的病毒家族，其天然宿主主要是哺乳动物和鸟类。到目前为止，已在人类中分离出13种PyVs，其中一些已明确与包括癌症在内的多种疾病相关。在本研究中，我们描述了使用灵敏的简并PCR方案结合新一代测序技术，从人类皮肤中分离出一种新型PyV的过程。这种新病毒名为里昂国际癌症研究机构多瘤病毒（LIPyV），具有一个5269个核苷酸的环状基因组。系统发育分析表明，LIPyV与在野生浣熊神经胶质瘤中鉴定出的浣熊PyV相关。对无癌个体的人类标本分析显示，9份皮肤拭子（9/445；2.0%）、3份咽漱液（3/140；2.1%）和1份眉毛毛发样本（1/439；0.2%）的LIPyV检测呈阳性。未来需要开展生物学和流行病学研究，以确认其对人类的嗜性，并深入了解其生物学特性。
