Wu Huansheng, Hou Chengyao, Wang Zhiru, Meng Puyan, Chen Hongying, Cao Huabin
Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang 330045, Jiangxi, PR China.
Zhengzhou Key Laboratory for Pig Disease Prevention and Control, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, PR China.
Vet Microbiol. 2022 Oct;273:109547. doi: 10.1016/j.vetmic.2022.109547. Epub 2022 Aug 17.
Porcine circovirus type 4 (PCV4), a unique circovirus with a different classification from other existing circovirus, was discovered in domestic pigs in several provinces of China. In this study, in order to investigate the epidemiology and genetic diversity of PCV4 in wild boars (Sus scrofa), a total number of 138 wild boar samples were collected from five different areas in Jiangxi Province of China, between January 2020 and December 2020. Taqman based real-time PCR were used to test PCV4 as well as PCV1, PCV2, and PCV3. Among 138 samples, 30 samples (21.7%) were positive for PCV1, 31 samples (22.5%) were positive for PCV2, 8 samples (5.8%) were positive for PCV3 and 27 samples (19.6%) were positive for PCV4, respectively. Some of the samples were co-infected with multiple PCVs. In this study, we successfully sequenced the complete genome of two PCV4 strains, which shared 98.5-99.8% of their genomic nucleotide similarity with the other five PCV4 strains discovered in domestic pigs. Phylogenetic analysis showed that the two PCV4 strains derived from wild boars were located in a closed relative branch with other PCV4 strains derived from domestic pigs, but were distinguished from other circovirus. These results of this study not only expand our understanding of the prevalence of PCVs, especially PCV4, in wild boars in Jiangxi province of China, but also showed the molecular epidemiology of PCV4. Nevertheless, the impact of wild boars infected with PCV4 on intensive farmed pigs industry remains to be further explored.
猪圆环病毒4型（PCV4）是一种独特的圆环病毒，其分类与其他现有圆环病毒不同，在中国多个省份的家猪中被发现。在本研究中，为了调查野猪（Sus scrofa）中PCV4的流行病学和遗传多样性，于2020年1月至2020年12月期间，从中国江西省五个不同地区共采集了138份野猪样本。采用基于Taqman的实时荧光定量PCR检测PCV4以及PCV1、PCV2和PCV3。在138份样本中，PCV1阳性样本30份（21.7%），PCV2阳性样本31份（22.5%），PCV3阳性样本8份（5.8%），PCV4阳性样本27份（19.6%）。部分样本存在多种猪圆环病毒的共感染情况。在本研究中，我们成功对两株PCV4毒株的全基因组进行了测序，这两株毒株与在家猪中发现的其他五株PCV4毒株的基因组核苷酸相似性为98.5 - 99.8%。系统发育分析表明，源自野猪的两株PCV4毒株与源自家猪的其他PCV4毒株位于一个紧密相关的分支中，但与其他圆环病毒区分开来。本研究结果不仅扩展了我们对中国江西省野猪中猪圆环病毒，尤其是PCV4流行情况的认识，还展示了PCV4的分子流行病学。然而，感染PCV4的野猪对集约化养殖猪产业的影响仍有待进一步探索。
